Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows.
Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows.
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DOI：
10.1038/s41598-018-24896-w
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发表时间：
2018-05-01
影响因子：
4.6
通讯作者：
de Paula SO
中科院分区：
文献类型：
--
作者：
da Silva Duarte V;Dias RS;Kropinski AM;Campanaro S;Treu L;Siqueira C;Vieira MS;da Silva Paes I;Santana GR;Martins F;Crispim JS;da Silva Xavier A;Ferro CG;Vidigal PMP;da Silva CC;de Paula SO
奶牛乳房炎仍然是奶农经济损失的主要原因。乳腺致病性大肠埃希菌(MPEC)与急性乳房炎有关，其治疗仍以抗生素为主。在抗菌素耐药(AMR)时代，细菌病毒(噬菌体)是一种有效的治疗或预防选择。然而，这使得它的遗传结构、稳定性以及与宿主免疫系统的相互作用得到彻底的表征是至关重要的。本研究从基因组的角度分析了一种新的、宿主范围广的抗乳房炎药物，T4病毒VB_Ecom-UFV13，以及它在实验性大肠杆菌诱导的乳房炎小鼠模型中对MPEC株的抑制作用。在VB_Ecom-UFV13和大肠杆菌噬菌体T4基因组之间分配了4975个单核苷酸多态(SNPs)，对病毒粒子蛋白的编码序列(CDS)有很高的影响(37.60%)。系统发育树和基因组分析支持最近VB_Ecom-UFV13和志贺氏菌噬菌体Shfl2之间的感染混合。在病毒稳定性评估(例如，pH和温度)后，乳房内给药(MOI 10)导致细菌负荷减少10倍。此外，在病毒治疗后观察到促炎细胞因子，如IL-6和肿瘤坏死因子-α。本工作对牛乳房炎的生物防治候选毒株VB_Ecom-UFV13进行了完整的鉴定和免疫应答。
Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.
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影响因子：
3.3
作者：
Dias, R. S.;Eller, M. R.;De Paula, S. O.
通讯作者：
De Paula, S. O.
影响因子：
5.2
作者：
Bryan D;El-Shibiny A;Hobbs Z;Porter J;Kutter EM
通讯作者：
Kutter EM
影响因子：
3.2
作者：
Déziel, E;Comeau, Y;Villemur, R
通讯作者：
Villemur, R
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
1.8
作者：
Brouillette, E;Grondin, G;Malouin, F
通讯作者：
Malouin, F