Discovery of Heteroaromatic Sulfones As a New Class of Biologically Compatible Thiol-Selective Reagents.
Discovery of Heteroaromatic Sulfones As a New Class of Biologically Compatible Thiol-Selective Reagents.
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DOI：
10.1021/acschembio.7b00444
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发表时间：
2017-08-18
影响因子：
4
通讯作者：
Furdui CM
中科院分区：
文献类型：
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作者：
Chen X;Wu H;Park CM;Poole TH;Keceli G;Devarie-Baez NO;Tsang AW;Lowther WT;Poole LB;King SB;Xian M;Furdui CM
化学试剂与还原的蛋白质硫醇的选择性反应是生物研究的关键。该反应用于防止常见蛋白质组学工作流程中含半胱氨酸肽的交联，并广泛应用于生理和病理过程机制的发现和靶向氧化还原研究。然而，发现已知和常用的硫醇封闭剂如碘乙酰胺、N-乙基马来酰亚胺等与氧化蛋白质次磺酸（-SOH）交叉反应，从而在采用这些试剂的研究中引入显著误差。我们研究并报道了一种新的杂芳烷基砜，4-（5-甲磺酰基-[1，2，3，4]四唑-1-基）-苯酚（MSTP），作为一种选择性和高活性的-SH封闭剂，与生物应用相容。
The selective reaction of chemical reagents with reduced protein thiols is critical to biological research. This reaction is utilized to prevent cross-linking of cysteine-containing peptides in common proteomics workflows and is applied widely in discovery and targeted redox investigations of the mechanisms underlying physiological and pathological processes. However, known and commonly used thiol blocking reagents like iodoacetamide, N-ethylmaleimide, and others were found to cross-react with oxidized protein sulfenic acids (–SOH) introducing significant errors in studies employing these reagents. We have investigated and are reporting here a new heteroaromatic alkylsulfone, 4-(5-methanesulfonyl-[1,2,3,4]tetrazol-1-yl)-phenol (MSTP), as a selective and highly reactive –SH blocking reagent compatible with biological applications.
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影响因子：
15
作者：
BRUICE, TC;SAYIGH, AB
通讯作者：
SAYIGH, AB
影响因子：
14.8
作者：
Peralta, David;Bronowska, Agnieszka K.;Dick, Tobias P.
通讯作者：
Dick, Tobias P.
影响因子：
6.6
作者：
Brandes N;Schmitt S;Jakob U
通讯作者：
Jakob U
影响因子：
14.8
作者：
Paulsen, Candice E.;Truong, Thu H.;Garcia, Francisco J.;Homann, Arne;Gupta, Vinayak;Leonard, Stephen E.;Carroll, Kate S.
通讯作者：
Carroll, Kate S.
影响因子：
21.8
作者：
通讯作者：
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