Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs.
Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs.
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单细胞图谱揭示了人类新生儿和成人椎间盘的细胞异质性和新标记。
DOI：
10.1016/j.isci.2022.104504
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发表时间：
2022-07-15
期刊：
影响因子：
5.8
通讯作者：
Sheyn D
中科院分区：
文献类型：
--
作者：
Jiang W;Glaeser JD;Salehi K;Kaneda G;Mathkar P;Wagner A;Ho R;Sheyn D
人类椎间盘（IVD）中细胞的起源、组成、分布和功能尚未完全了解。在这里，已经生成了人类新生儿和成人IVD的细胞图谱，并通过基因本体论途径富集、伪时间轨迹、组织学和免疫荧光进行了进一步评估。细胞图谱的比较显示，存在两个亚群的脊索细胞（NC）及其相关的标记物在新生儿和成人IVD。发育轨迹预测了7种不同的细胞状态，描述了IVD从新生细胞到成体细胞的发育过程，并分析了NC在IVD发育中的作用。在新生儿IVD中检测到纤维环细胞（AFC）的高度异质性和逐渐转变，并评估了其在IVD发展中的潜在相关性。总的来说，比较新生儿和成人IVD之间的单细胞图谱描绘了IVD细胞生物学的景观，并可能有助于发现IVD变性的新治疗靶点。比较人新生儿和成人IVD之间的scRNA-seq鉴定了成人中的两个脊索细胞群体及其新标记物脊索细胞将其身份和功能保留到成年期揭示了人IVD中髓核和纤维环细胞的异质性复杂系统生物学;发育生物学;转录组学
The origin, composition, distribution, and function of cells in the human intervertebral disc (IVD) have not been fully understood. Here, cell atlases of both human neonatal and adult IVDs have been generated and further assessed by gene ontology pathway enrichment, pseudo-time trajectory, histology, and immunofluorescence. Comparison of cell atlases revealed the presence of two subpopulations of notochordal cells (NCs) and their associated markers in both the neonatal and adult IVDs. Developmental trajectories predicted 7 different cell states that describe the developmental process from neonatal to adult cells in IVD and analyzed the NC’s role in the IVD development. A high heterogeneity and gradual transition of annulus fibrosus cells (AFCs) in the neonatal IVD was detected and their potential relevance in IVD development assessed. Collectively, comparing single-cell atlases between neonatal and adult IVDs delineates the landscape of IVD cell biology and may help discover novel therapeutic targets for IVD degeneration. Compared scRNA-seq between human neonatal and adult IVD Identified two notochordal cell populations in adults and their novel markers Notochordal cells preserved their identity and functions into adulthood Unveiled heterogeneity of nucleus pulposus and annulus fibrosus cells in human IVD Complex system biology; Developmental biology; Transcriptomics
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影响因子：
6.9
作者：
Kelly, Natalie H.;Huynh, Nguyen P. T.;Guilak, Farshid
通讯作者：
Guilak, Farshid
影响因子：
3
作者：
Kondo N;Yuasa T;Shimono K;Tung W;Okabe T;Yasuhara R;Pacifici M;Zhang Y;Iwamoto M;Enomoto-Iwamoto M
通讯作者：
Enomoto-Iwamoto M
影响因子：
2.8
作者：
Pratsinis, Harris;Kletsas, Dimitris
通讯作者：
Kletsas, Dimitris
IncRNA：椎间盘退变和骨关节炎的新参与者
影响因子：
8.5
作者：
Chen WK;Yu XH;Yang W;Wang C;He WS;Yan YG;Zhang J;Wang WJ
通讯作者：
Wang WJ
影响因子：
3.1
作者：
Johnson ZI;Schoepflin ZR;Choi H;Shapiro IM;Risbud MV
通讯作者：
Risbud MV