Analysis of Gene Expression Responses to a Salmonella Infection in Rugao Chicken Intestine Using GeneChips.
Analysis of Gene Expression Responses to a Salmonella Infection in Rugao Chicken Intestine Using GeneChips.
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使用基因芯片分析如皋鸡肠道对沙门氏菌感染的基因表达反应
DOI：
10.5713/ajas.2011.11174
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Chen GH
中科院分区：
文献类型：
--
作者：
Luan DQ;Chang GB;Sheng ZW;Zhang Y;Zhou W;Li ZZ;Liu Y;Chen GH
家禽产品是肠道沙门氏菌的重要来源。减少由沙门氏菌引起的食物中毒的一个有效方法是培育更耐感染的鸡。不幸的是，宿主对沙门氏菌的反应是复杂的，涉及到许多因素。为了更好地了解2周龄雏鸡对沙门氏菌的反应，应用包含13,319个探针的基因芯片比较了两组鸡在对照和沙门氏菌感染条件下的基因表达谱。用肠炎沙门氏菌口服感染初孵化雏鸡。由于肠道是细菌在口服接种后遇到的重要屏障之一，因此在2周龄时对肠道基因的表达进行了研究。共检测到差异表达基因588个，其中已知基因276个，上调表达基因266个，下调表达基因322个。在对照组和沙门氏菌感染条件下，两组鸡的基因表达存在差异，表明两组鸡的肠道发育存在差异，这可能与沙门氏菌敏感性的差异有关。通过实时定量聚合酶链式反应证实了4个基因的差异表达，结果表明这些基因的表达变化与GeneChips的结果基本一致。这项研究中的发现导致了新基因和可能的细胞通路的识别，这些基因和可能的细胞通路依赖于宿主。
Poultry products are an important source of Salmonella enterica. An effective way to reduce food poisoning due to Salmonella would be to breed chickens more resistant to infection. Unfortunately host responses to Salmonella are complex with many factors involved. To learn more about responses to Salmonella in young chickens of 2 wk old, a cDNA Microarray containing 13,319 probes was performed to compare gene expression profiles between two chicken groups under control and Salmonella infected conditions. Newly hatched chickens were orally infected with S. enterica serovar Enteritidis. Since the intestine is one of the important barriers the bacteria encounter after oral inoculation, intestine gene expression was investigated at 2 wk old. There were 588 differentially expressed genes detected, of which 276 were known genes, and of the total number 266 were up-regulated and 322 were down-regulated. Differences in gene expression between the two chicken groups were found in control as well as Salmonella infected conditions indicating a difference in the intestine development between the two chicken groups which might be linked to the difference in Salmonella susceptibility. The differential expressions of 4 genes were confirmed by quantitative real-time PCR and the results indicated that the expression changes of these genes were generally consistent with the results of GeneChips. The findings in this study have lead to the identification of novel genes and possible cellular pathways, which are host dependent.
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影响因子：
4.4
作者：
Zeng, H;Carlson, AQ;Neish, AS
通讯作者：
Neish, AS
DOI：
10.1016/s1096-4959(01)00389-x
发表时间：
2001-07-01
影响因子：
2.2
作者：
Amundson, SA;Bittner, M;Fornace, AJ
通讯作者：
Fornace, AJ
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
2.4
作者：
BARROW, PA;HUGGINS, MB;SIMPSON, JM
通讯作者：
SIMPSON, JM
影响因子：
2.7
作者：
Shin, Masahiro;Noji, Surnihare;Yasugi, Sadao
通讯作者：
Yasugi, Sadao