Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs in vitro models of mesothelioma.
Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs in vitro models of mesothelioma.
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DOI：
10.1183/13993003.00143-2023
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发表时间：
2024-01
影响因子：
24.3
通讯作者：
Marciniak, Stefan J.
中科院分区：
文献类型：
--
作者：
Obacz, Joanna;Valer, Jose Antonio;Nibhani, Reshma;Adams, Taylor S.;Schupp, Jonas C.;Veale, Niki;Lewis-Wade, Amanah;Flint, Jasper;Hogan, John;Aresu, Giuseppe;Coonar, Aman S.;Peryt, Adam;Biffi, Giulia;Kaminski, Naftali;Francies, Hayley;Rassl, Doris M.;Garnett, Mathew J.;Rintoul, Robert C.;Marciniak, Stefan J.
胸腔的胸膜衬里调节局部免疫、炎症和修复。各种情况下，良性和恶性，包括胸膜间皮瘤，可以影响这一组织。缺乏知识的间皮细胞和基质细胞组成的胸膜，阻碍了发展的靶向治疗。在这里，我们提出了第一个全面的人类壁层胸膜单细胞转录组图谱，并证明其在阐明胸膜生物学的效用。我们确认存在已知的通用成纤维细胞，并描述了新的，潜在的胸膜特异性，成纤维细胞亚型。我们还提出了多种良性和恶性间皮细胞体外模型的转录组学特征，并通过与体内转录组学数据的比较来验证这些特征。虽然之前已经报道了大量胸膜转录组，但这是第一项在单细胞水平上提供分辨率的研究。我们希望我们的胸膜细胞图谱对研究胸膜生物学和疾病的人来说是无价的。它已经使我们能够阐明间皮细胞的转分化，使我们能够开发一种简单的方法，延长间皮细胞的分化在体外。人类壁层胸膜的第一个单细胞RNA-seq图谱现在可以通过免费的基于网络的数据挖掘工具MesothelialCellAtlas.com访问，并阐明了良性和恶性胸膜生物学的各个方面https://bit.ly/3u3eDu8
The pleural lining of the thorax regulates local immunity, inflammation and repair. A variety of conditions, both benign and malignant, including pleural mesothelioma, can affect this tissue. A lack of knowledge concerning the mesothelial and stromal cells comprising the pleura has hampered the development of targeted therapies. Here, we present the first comprehensive single-cell transcriptomic atlas of the human parietal pleura and demonstrate its utility in elucidating pleural biology. We confirm the presence of known universal fibroblasts and describe novel, potentially pleural-specific, fibroblast subtypes. We also present transcriptomic characterisation of multiple in vitro models of benign and malignant mesothelial cells, and characterise these through comparison with in vivo transcriptomic data. While bulk pleural transcriptomes have been reported previously, this is the first study to provide resolution at the single-cell level. We expect our pleural cell atlas will prove invaluable to those studying pleural biology and disease. It has already enabled us to shed light on the transdifferentiation of mesothelial cells, allowing us to develop a simple method for prolonging mesothelial cell differentiation in vitro. The first single-cell RNA-seq atlas of human parietal pleura is now accessible via a free web-based data-mining tool, MesothelialCellAtlas.com, and illuminates aspects of benign and malignant pleural biology https://bit.ly/3u3eDu8
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
间皮细胞分化为成骨细胞和脂肪细胞样细胞。
影响因子：
5.3
作者：
Lansley SM;Searles RG;Hoi A;Thomas C;Moneta H;Herrick SE;Thompson PJ;Newman M;Sterrett GF;Prêle CM;Mutsaers SE
通讯作者：
Mutsaers SE
影响因子：
46.9
作者：
Moon, Kevin R.;van Dijk, David;Krishnaswamy, Smita
通讯作者：
Krishnaswamy, Smita
影响因子：
48
作者：
Browaeys, Robin;Saelens, Wouter;Saeys, Yvan
通讯作者：
Saeys, Yvan
影响因子：
28.2
作者：
Elyada, Ela;Bolisetty, Mohan;Tuveson, David A.
通讯作者：
Tuveson, David A.