Murine Sperm Expresses Toll-Like Receptor (TLR) Family that Responds to the Pathogens Released from Virus, and Decreases Fertilization Ability by the Stimuli
Murine Sperm Expresses Toll-Like Receptor (TLR) Family that Responds to the Pathogens Released from Virus, and Decreases Fertilization Ability by the Stimuli
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小鼠精子表达 Toll 样受体 (TLR) 家族，该家族对病毒释放的病原体做出反应，并通过刺激降低受精能力
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Shimada
中科院分区：
文献类型：
--
作者：
Toshihiro Mihara;Keita Fukumoto;T. Okazaki;M. Shimada
摘要：精子表达Toll样受体（TLR）家族，具有防御精液细菌感染的先天免疫功能。已知在精子活力低的精液中也观察到病毒感染，这表明精子也能够对病毒感染做出反应。然而，关于识别精液中病毒释放的病原体的 TLR 的表达和作用的信息很少。本研究清楚地表明精子表达功能性 TLR9，可识别未甲基化的 CpG DNA 序列。使用特异性抗体，我们检测了精子中TLR9的表达。然而，通过诱导顶体反应的黄体酮处理，阳性条带消失。当精子与 TLR9 配体一起培养时，精子活力以时间依赖性方式显着下降。用 TLR9 配体处理精子会影响顶体状态并抑制 BSA 诱导的精子获能。此外，在用 TLR9 配体预培养后，精子被用于体外受精并注射到超排卵雌性小鼠的子宫中。体外和体内的受精率均显着受到抑制。从这些结果中，我们得出结论，精子表达并具有功能性 TLR9，可以识别精液中的病毒感染。
Abstract: Sperm express the Toll-like receptor (TLR) family and have an innate immune function defending against bacterial infection in semen. It has been known that virus infection is also observed in semen with low motility of sperm, suggesting that sperm are also able to respond to virus infection. However, there is little information about the expression and roles of TLRs that recognize virus-released pathogens in semen. The present study clearly showed that sperm express functional TLR9 that recognizes the unmethylated CpG DNA sequence. Using a specific antibody, we detected the expression of TLR9 in sperm. However, the positive band was disappeared by progesterone treatment that induced the acrosome reaction. When sperm were cultured with the TLR9 ligand, sperm motility significantly decreased in a time-dependent manner. The treatment of sperm with the TLR9 ligand affected the acrosomal status and suppressed the BSA-induced capacitation of sperm. Additionally, sperm were used for in vitro fertilization and injection into the uterus of super-ovulated female mice following pre-culture with the TLR9 ligand. The fertilization rates were significantly suppressed both in vitro and in vivo. From these results, we conclude that sperm express and have functional TLR9 that potentially recognizes virus infection in semen.
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