A monoclonal antibody toolkit for C. elegans.
A monoclonal antibody toolkit for C. elegans.
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DOI：
10.1371/journal.pone.0010161
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发表时间：
2010-04-13
期刊：
影响因子：
3.7
通讯作者：
Nonet ML
中科院分区：
文献类型：
--
作者：
Hadwiger G;Dour S;Arur S;Fox P;Nonet ML
抗体是许多生物学研究领域的重要工具。虽然抗体可以在研究实验室环境中产生，但大多数研究脊椎动物的研究实验室都利用各种各样的市售试剂。相比之下，很少有这样的试剂可用于与模式生物的工作。我们报告生产的单克隆抗体针对广泛的蛋白质，标签特定的亚细胞和细胞成分，和大分子复合物。制备了针对突触泡蛋白（SNB-1）、Rim（Rim-10）、转化酸性卷曲螺旋蛋白（TAC-1）、CENP-C（HCP-4）、ORC 2（ORC-2）、CENP-C（HCP-4）的抗体，SNB-1是突触小泡的一种组分，Rim-10是位于突触活性区的一种蛋白，TAC-1是中心体的一种组分，CENP-C（HCP-4）在蠕虫中标记其全着丝粒染色体的全长，ORC 2是DNA起点复制复合物的一个亚基，ORC-2是一种蛋白质。核仁磷蛋白NOPP 140（DAO-5）;核膜蛋白核纤层蛋白（LMN-1）; EHD 1（RME-1），一种内体再循环的标记物;小窝蛋白（CAV-1），一种小窝的标记物;细胞色素P450（α-33 E1），内质网的居民;标记高尔基体的β-1，3-葡糖醛酸转移酶（SQV-8）;靶向线粒体的伴侣蛋白（HSP-60）; LAMP（LMP-1），一种溶酶体的常驻蛋白; 26 S蛋白酶体的20 S亚复合物的α亚基（PAS-7）;发动蛋白（DYN-1）和衔接子复合物2的α亚基（阿帕-2），作为网格蛋白介导的内吞作用位点的标记物; MAGUK，蛋白质盘大（DLG-1）和钙粘蛋白（HMR-1），两者都标记粘附连接;定位于顶膜的埃兹蛋白-根蛋白-膜突蛋白家族（ERM-1）的细胞骨架接头;定位于上皮细胞基底外侧膜的ERBIN家族蛋白（LET-413）和在细胞-细胞接触时定位于质膜的粘附分子（SAX-7）。除了在整体免疫细胞化学中起作用外，这些抗体中的大多数在蛋白质印迹中起作用，因此应该用于生化分级研究。我们已经制备了一组抗线虫C亚细胞组分的单克隆抗体。elegans为研究社区。这些试剂通过发育研究杂交库（DSHB）提供。
Antibodies are critical tools in many avenues of biological research. Though antibodies can be produced in the research laboratory setting, most research labs working with vertebrates avail themselves of the wide array of commercially available reagents. By contrast, few such reagents are available for work with model organisms. We report the production of monoclonal antibodies directed against a wide range of proteins that label specific subcellular and cellular components, and macromolecular complexes. Antibodies were made to synaptobrevin (SNB-1), a component of synaptic vesicles; to Rim (UNC-10), a protein localized to synaptic active zones; to transforming acidic coiled-coil protein (TAC-1), a component of centrosomes; to CENP-C (HCP-4), which in worms labels the entire length of their holocentric chromosomes; to ORC2 (ORC-2), a subunit of the DNA origin replication complex; to the nucleolar phosphoprotein NOPP140 (DAO-5); to the nuclear envelope protein lamin (LMN-1); to EHD1 (RME-1) a marker for recycling endosomes; to caveolin (CAV-1), a marker for caveolae; to the cytochrome P450 (CYP-33E1), a resident of the endoplasmic reticulum; to β-1,3-glucuronyltransferase (SQV-8) that labels the Golgi; to a chaperonin (HSP-60) targeted to mitochondria; to LAMP (LMP-1), a resident protein of lysosomes; to the alpha subunit of the 20S subcomplex (PAS-7) of the 26S proteasome; to dynamin (DYN-1) and to the α-subunit of the adaptor complex 2 (APA-2) as markers for sites of clathrin-mediated endocytosis; to the MAGUK, protein disks large (DLG-1) and cadherin (HMR-1), both of which label adherens junctions; to a cytoskeletal linker of the ezrin-radixin-moesin family (ERM-1), which localized to apical membranes; to an ERBIN family protein (LET-413) which localizes to the basolateral membrane of epithelial cells and to an adhesion molecule (SAX-7) which localizes to the plasma membrane at cell-cell contacts. In addition to working in whole mount immunocytochemistry, most of these antibodies work on western blots and thus should be of use for biochemical fractionation studies. We have produced a set of monoclonal antibodies to subcellular components of the nematode C. elegans for the research community. These reagents are being made available through the Developmental Studies Hybridoma Bank (DSHB).
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影响因子：
2.5
作者：
Gracheva EO;Hadwiger G;Nonet ML;Richmond JE
通讯作者：
Richmond JE
DOI：
10.1083/jcb.101.4.1532
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Francis GR;Waterston RH
通讯作者：
Waterston RH
影响因子：
56.9
作者：
GOSSEN, M;PAK, DTS;BOTCHAN, MR
通讯作者：
BOTCHAN, MR
影响因子：
56.9
作者：
FOSS, M;MCNALLY, FJ;RINE, J
通讯作者：
RINE, J
影响因子：
4.6
作者：
Han, Sung Min;Lee, Tae Hoon;Koo, Hyeon-Sook
通讯作者：
Koo, Hyeon-Sook