Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.
Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.
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抗SARS-CoV-2变异株双价中和抗体的结构和功能分析
DOI：
10.1016/j.isci.2022.104431
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发表时间：
2022-06-17
期刊：
影响因子：
5.8
通讯作者：
Zhang, Zheng
中科院分区：
文献类型：
--
作者：
Li, Yaning;Fan, Qing;Zhou, Bing;Shen, Yaping;Zhang, Yuanyuan;Cheng, Lin;Qi, Furong;Song, Shuo;Guo, Yingying;Yan, Renhong;Ju, Bin;Zhang, Zheng
严重急性呼吸道综合征冠状病毒2型（SARS-CoV-2）的不同变体引起了公众的最大关注，因为它们引起了COVID-19的“一波又一波”大流行。病毒感染的初始步骤由SARS-CoV-2 Spike（S）蛋白介导，该蛋白介导病毒与宿主细胞之间的受体识别和膜融合。针对SARS-CoV-2 S蛋白的中和抗体（nAbs）已成为临床干预策略的有希望的候选者，而多项研究表明，不同的变体具有增强的感染性和抗体抗性。在此，我们探讨了STS 165的结构和功能，STS 165是一种针对SARS-CoV-2变体甚至SARS-CoV的广泛的刺突间双价nAb，有助于进一步理解nAb的工作机制。STS 165广泛中和SARS-CoV-2的不同变体，甚至SARS-CoV STS 165显示刺突间二价结合特性STS 165可以作为形成治疗性抗体鸡尾酒的理想伴侣生物化学应用;分子结构;结构生物学;病毒学
The different variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have attracted most public concern because they caused “wave and wave” COVID-19 pandemic. The initial step of viral infection is mediated by the SARS-CoV-2 Spike (S) protein, which mediates the receptor recognition and membrane fusion between virus and host cells. Neutralizing antibodies (nAbs) targeting the S protein of SARS-CoV-2 have become promising candidates for clinical intervention strategy, while multiple studies have shown that different variants have enhanced infectivity and antibody resistance. Here, we explore the structure and function of STS165, a broadly inter-Spike bivalent nAb against SARS-CoV-2 variants and even SARS-CoV, contributing to further understanding of the working mechanism of nAbs. STS165 broadly neutralizes different variants of SARS-CoV-2 and even SARS-CoV STS165 exhibits inter-Spike bivalent binding characteristics STS165 may work as an ideal partner to form therapeutic antibody cocktails Biochemistry applications; Molecular Structure; Structural biology; Virology
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影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
电子计数和束诱导运动校正可实现近原子分辨率的单粒子冷冻电镜。
影响因子：
48
作者：
Li, Xueming;Mooney, Paul;Zheng, Shawn;Booth, Christopher R.;Braunfeld, Michael B.;Gubbens, Sander;Agard, David A.;Cheng, Yifan
通讯作者：
Cheng, Yifan
影响因子：
64.5
作者：
Liu C;Ginn HM;Dejnirattisai W;Supasa P;Wang B;Tuekprakhon A;Nutalai R;Zhou D;Mentzer AJ;Zhao Y;Duyvesteyn HME;López-Camacho C;Slon-Campos J;Walter TS;Skelly D;Johnson SA;Ritter TG;Mason C;Costa Clemens SA;Gomes Naveca F;Nascimento V;Nascimento F;Fernandes da Costa C;Resende PC;Pauvolid-Correa A;Siqueira MM;Dold C;Temperton N;Dong T;Pollard AJ;Knight JC;Crook D;Lambe T;Clutterbuck E;Bibi S;Flaxman A;Bittaye M;Belij-Rammerstorfer S;Gilbert SC;Malik T;Carroll MW;Klenerman P;Barnes E;Dunachie SJ;Baillie V;Serafin N;Ditse Z;Da Silva K;Paterson NG;Williams MA;Hall DR;Madhi S;Nunes MC;Goulder P;Fry EE;Mongkolsapaya J;Ren J;Stuart DI;Screaton GR
通讯作者：
Screaton GR
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH