Competitive assembly to increase the performance of the DNA/carbon-nanomaterial-based sensing platform.
Competitive assembly to increase the performance of the DNA/carbon-nanomaterial-based sensing platform.
复制标题
竞争性组装可提高基于 DNA/碳纳米材料的传感平台的性能。
DOI：
10.1021/am502311b
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Zheng, Jing;Li, Yinhui;Yang, Ronghua;Tan, Weihong
提高表面目标结合率和增强荧光信号恢复效率对于碳纳米材料（例如单壁碳纳米管（SWNT））的理想生物医学应用至关重要。我们在此描述了一种使用短互补 DNA (scDNA) 链提高 DNA 功能化 SWNT 表面上的靶标结合率并增强荧光信号恢复效率的策略。 scDNA 通过在纳米结构表面竞争性组装并诱导构象变化使 DNA 远离表面，从而使关联率提高 2.5 倍，荧光信号恢复提高 4 倍，使其更容易结合靶核酸。 scDNA 诱导的结合动力学和荧光信号恢复效率的增强是所有研究的序列和表面都会发生的普遍现象。通过这种竞争性的 scDNA 组装策略，可以提高基于碳纳米材料的生物传感平台的体外检测和活细胞成像的性能。
Increasing the rate of target binding on the surface and enhancing the fluorescence signal restoration efficiency are critical to the desirable biomedical application of carbon nanomaterials, for example, single-walled carbon nanotubes (SWNTs). We describe here a strategy to increase the target binding rate and enhance the fluorescence signal restoration efficiency on the DNA-functionalized SWNT surface using a short complementary DNA (scDNA) strand. The scDNA causes up to a 2.5-fold increase in association rate and 4-fold increase in fluorescence signal restoration by its competitive assembly on the nanostructure's surface and inducing a conformational change that extends the DNA away from the surface, making it more available to bind target nucleic acids. The scDNA-induced enhancement of binding kinetics and fluorescence signal restoration efficiency is a general phenomenon that occurred with all sequences and surfaces investigated. Through this competitive assembly strategy of scDNA, performance improvement of the carbon-nanomaterial-based biosensing platform for both in vitro detection and live cell imaging can be reached.
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