Fluorescent probes for nucleic Acid visualization in fixed and live cells.
Fluorescent probes for nucleic Acid visualization in fixed and live cells.
复制标题
固定细胞和活细胞中核酸可视化的荧光探针。
DOI：
10.3390/molecules181215357
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发表时间：
2013-12-11
期刊：
影响因子：
--
通讯作者：
Venyaminova AG
中科院分区：
文献类型：
--
作者：
Boutorine AS;Novopashina DS;Krasheninina OA;Nozeret K;Venyaminova AG
本文从非荧光探针和荧光探针对细胞内天然RNA和DNA成像的适用性角度，对固定细胞和活细胞核酸成像的相关文献进行了分析。荧光显微镜成像主要关注荧光探针。制定了对目标结合部分和组成探针的荧光团的要求。在原生双链DNA的情况下，结构特异性和序列特异性探针进行了讨论。在最新的研究中，描述了三类ddna靶向分子：(i)序列特异性肽和蛋白质；（ii）形成三聚体的寡核苷酸和（iii）聚酰胺寡聚（n -甲基吡咯/ n -甲基咪唑）次要凹槽粘合剂。聚酰胺似乎是最有前途的荧光探针设计靶向剂，但其序列特异性较低、细胞内背景荧光高等技术问题仍有待解决。荧光探针应用于DNA成像在固定细胞和活细胞的几个例子被引用。在细胞内RNA的情况下，只有修饰的寡核苷酸才能提供这种序列特异性成像。考虑了几种设计荧光探针的方法：基于修饰的寡核苷酸类似物的线性荧光探针、分子信标、二元荧光探针和荧光探针形成的模板定向反应、FRET供体-受体对、芘准分子、适体等。讨论了所有这些方法在活细胞应用中的适用性。
This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to fluorescent probes for fluorescence microscopy imaging. Requirements for the target-binding part and the fluorophore making up the probe are formulated. In the case of native double-stranded DNA, structure-specific and sequence-specific probes are discussed. Among the latest, three classes of dsDNA-targeting molecules are described: (i) sequence-specific peptides and proteins; (ii) triplex-forming oligonucleotides and (iii) polyamide oligo(N-methylpyrrole/N-methylimidazole) minor groove binders. Polyamides seem to be the most promising targeting agents for fluorescent probe design, however, some technical problems remain to be solved, such as the relatively low sequence specificity and the high background fluorescence inside the cells. Several examples of fluorescent probe applications for DNA imaging in fixed and living cells are cited. In the case of intracellular RNA, only modified oligonucleotides can provide such sequence-specific imaging. Several approaches for designing fluorescent probes are considered: linear fluorescent probes based on modified oligonucleotide analogs, molecular beacons, binary fluorescent probes and template-directed reactions with fluorescence probe formation, FRET donor-acceptor pairs, pyrene excimers, aptamers and others. The suitability of all these methods for living cell applications is discussed.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
DOI：
10.1073/pnas.2233244100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bratu, DP;Cha, BJ;Tyagi, S
通讯作者：
Tyagi, S
影响因子：
9.2
作者：
Beach, DL;Salmon, ED;Bloom, K
通讯作者：
Bloom, K
影响因子：
56.9
作者：
Boch, Jens;Scholze, Heidi;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
14.9
作者：
Chan FL;Wong LH
通讯作者：
Wong LH