Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity.
Optogenetic drive of neocortical pyramidal neurons generates fMRI signals that are correlated with spiking activity.
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DOI：
10.1016/j.brainres.2013.03.011
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发表时间：
2013-05-20
期刊：
影响因子：
2.9
通讯作者：
Moore CI
中科院分区：
文献类型：
--
作者：
Kahn I;Knoblich U;Desai M;Bernstein J;Graybiel AM;Boyden ES;Buckner RL;Moore CI
血氧水平依赖（BOLD）信号的局部波动是功能磁共振成像（fMRI）的基础。理解神经活动的不同方面和BOLD反应之间的相关性是解释这种广泛使用的映射信号的基础。对这个问题的分析需要能够精确地操纵定义的神经元的活动。为了实现这样的控制，我们将新皮层神经元的光遗传学驱动与高分辨率（9.4 T）啮齿动物fMRI和神经生理数据的详细分析相结合。光驱动的锥体神经元的激活导致在刺激部位的阳性BOLD反应。为了帮助区分BOLD响应的神经生理学相关性，我们采用了具有相同平均频率但具有周期性和泊松分布脉冲时间的光列。这些不同类型的脉冲序列产生了单单位、多单位和局部场电位（LFP）活动以及BOLD信号的可分离模式。BOLD活动表现出最强的相关性尖峰活动与刺激率的增加，并在第一近似，是线性的脉冲输送率，而LFP活动表现出较弱的相关性。这些数据提供了尖峰频率和BOLD响应之间强相关性的示例。
Local fluctuations in the blood oxygenation level-dependent (BOLD) signal serve as the basis of functional magnetic resonance imaging (fMRI). Understanding the correlation between distinct aspects of neural activity and the BOLD response is fundamental to the interpretation of this widely used mapping signal. Analysis of this question requires the ability to precisely manipulate the activity of defined neurons. To achieve such control, we combined optogenetic drive of neocortical neurons with high-resolution (9.4 T) rodent fMRI and detailed analysis of neurophysiological data. Light-driven activation of pyramidal neurons resulted in a positive BOLD response at the stimulated site. To help differentiate the neurophysiological correlate(s) of the BOLD response, we employed light trains of the same average frequency, but with periodic and Poisson distributed pulse times. These different types of pulse trains generated dissociable patterns of single-unit, multi-unit and local field potential (LFP) activity, and of BOLD signals. The BOLD activity exhibited the strongest correlation to spiking activity with increasing rates of stimulation, and, to a first approximation, was linear with pulse delivery rate, while LFP activity showed a weaker correlation. These data provide an example of a strong correlation between spike rate and the BOLD response.
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