A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense.
A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense.
复制标题
斑马鱼斑马鱼的一种新型肝凝集素参与先天免疫防御。
DOI：
10.1016/j.fsi.2019.10.068
复制
发表时间：
2020-03
影响因子：
4.7
通讯作者：
Li Hongyan
中科院分区：
文献类型：
--
作者：
Yang Qingyun;Wang Peng;Yang Shuaiqi;Li Xianpeng;Zhang Xiangmin;Ji Guangdong;Zhang Shicui;Wang Su;Li Hongyan
ASGPR（asialoglycoprotein receptor，脱唾液酸糖蛋白受体，又称肝凝集素）是最早发现的动物凝集素，参与多种生理过程。然而，其详细的免疫功能在低等脊椎动物中没有得到很好的研究。在报道了一种斑马鱼肝凝集素（Zebrafish hepatic lectin，Zhl）后，我们在斑马鱼中鉴定了一种新的肝凝集素（zebrafish hepatic lectin-like，Zhl-l）。该基因主要在肝脏中表达，并具有组织特异性。LPS/LTA刺激后，Zh 1表达明显改变。此外，Zhl-1的重组C型凝集素结构域（rCTLD）对革兰氏阴性菌和革兰氏阳性菌均具有凝集和结合活性。促进鲤鱼巨噬细胞对细菌的吞噬。rCTLD与脂多糖（LPS）、脂磷壁酸（LTA）和肽聚糖（PGN）的结合不依赖于Ca ~（2+），但可被半乳糖抑制。有趣的是，Zhl-1位于膜上，并且其过表达可以上调炎症前细胞因子的产生。这些结果表明，Zhl-1在免疫防御中发挥重要作用，为进一步了解C型凝集素家族的功能和脊椎动物的天然免疫提供了信息。
ASGPR (asialoglycoprotein receptor, also known as hepatic lectin) was the first identified animal lectin, which participated in a variety of physiological processes. Yet its detailed immune functions are not well studied in lower vertebrates. After reporting a zebrafish hepatic lectin (Zhl), we identified a novel hepatic lectin (zebrafish hepatic lectin-like, Zhl-l) in zebrafish. Thezhl-lwas mainly expressed in liver in a tissue specific manner. And challenge with LPS/LTA induced a significant change ofzhl-lexpression. What's more, recombinant C-type lectin domain (rCTLD) of Zhl-l had the activity of agglutinating and binding to both Gram-negative and Gram-positive bacteria. It promoted the phagocytosis of bacteria by carp macrophages. Moreover, rCTLD could bind to insoluble lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN) independent of Ca2+, which was inhibited by galactose. Interestingly, Zhl-l was located in the membrane, and its overexpression could upregulate the production of pre-inflammatory cytokines. Taken together, these results indicated that Zhl-l played a role in immune defense, and would provide further information to understand functions of C-type lectin family and the innate immunity in vertebrates.
登录
查看更多内容
影响因子：
2.9
作者：
Shu C;Wang S;Xu T
通讯作者：
Xu T
影响因子：
2.8
作者：
U. Holmskov
通讯作者：
U. Holmskov
影响因子：
3.5
作者：
Qu, Baozhen;Yang, Shuangshuang;Zhang, Shicui
通讯作者：
Zhang, Shicui
影响因子：
3.6
作者：
Sun, Yun-Dong;Fu, Li-Dong;Wang, Jin-Xing
通讯作者：
Wang, Jin-Xing
影响因子：
4
作者：
Liu, Mingying;Zhang, Shicui
通讯作者：
Zhang, Shicui