Association Between Programed Cell Death-1 and CD4(+) T Cell Alterations in Different Phases of Ischemic Stroke Patients.
Association Between Programed Cell Death-1 and CD4(+) T Cell Alterations in Different Phases of Ischemic Stroke Patients.
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缺血性脑卒中患者不同阶段程序性细胞死亡-1和CD4(+)T细胞改变之间的关联
DOI：
10.3389/fncel.2018.00170
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发表时间：
2018
影响因子：
5.3
通讯作者：
Yuan Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Wei L;Du Y;Xie Y;Wu W;Yuan Y
目的：我们旨在分析缺血性脑卒中（IS）不同阶段T细胞亚群的变化，以探索脑卒中诱导的免疫抑制（SIID）的潜在可能机制。
方法：64例符合入选标准的缺血性脑卒中患者被分为三组：急性期组、亚急性期组和稳定期组。选取14名健康个体作为正常对照。分析患者外周血中T细胞的表型分布，并检测不同T细胞表型中的免疫检查点受体程序性细胞死亡 - 1（PD - 1）和T细胞免疫球蛋白及黏蛋白结构域3（Tim - 3）。
结果：与对照组相比，急性期组CD4⁺T细胞和CD4⁺T中央记忆（TCM）细胞的绝对数量显著增加，但亚急性期组和稳定期组与急性期组相比则减少。稳定期组CD4⁺T细胞中PD - 1的表达与急性期组相比显著增加。急性期CD4⁺TCM细胞和CD4⁺T效应记忆（TEM）细胞上PD - 1的表达与对照细胞相比显著降低；然而，在亚急性期和稳定期，PD - 1的表达与急性期相比显著增加。
结论：在缺血性脑卒中的不同阶段发生了T细胞功能障碍，尤其是CD4⁺T细胞功能障碍。急性期后不同表型的CD4⁺T细胞中PD - 1高表达，且与CD4⁺T细胞的变化相关。特别是，在不同CD4⁺T细胞表型中，PD - 1与TCM细胞的绝对数量呈负相关，这可能是SIID的可能机制之一。
Objective: We aimed to analyze alterations in T cell subgroups during different post-ischemic stroke (IS) phases to explore the possible mechanisms underlying stroke-induced immune depression (SIID). Methods: Sixty-four IS patients who met the entry criteria were divided into three groups: an acute phase group, a sub-acute phase group and a stable phase group. Fourteen healthy individuals were selected as normal controls. The phenotype distribution of T cells in patient peripheral blood was analyzed, and the immune checkpoint receptors programed cell death-1 (PD-1) and T cell immunoglobulin and mucin domain 3 (Tim-3) were detected in different T cell phenotypes. Results: Compared with the control group, the absolute number of CD4+ T cells and CD4+ T central memory (TCM) cells was significantly increased in the acute phase group but decreased in the sub-acute phase and stable phase groups compared with that in the acute phase group. PD-1 expression in CD4+ T cells in the stable phase group showed a significant increase compared with that in the acute phase group. The expression of PD-1 on CD4+ TCM cells and CD4+ T effector memory (TEM) cells showed significant decreases in the acute phase compared with control cells; however, in the sub-acute phase and the stable phase, PD-1 expression was significantly increased compared with that in the acute phase. Conclusions: T cell dysfunction, especially CD4+ T cell dysfunction, occurred during different IS phases. PD-1 was highly expressed in CD4+ T cells of different phenotypes after the acute phase and was associated with alterations in CD4+ T cells. Particularly, PD-1 was negatively correlated with the absolute number of TCM cells among different CD4+ T cell phenotypes, which may be one of the possible mechanisms of SIID.
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影响因子：
9.3
作者：
Bodhankar S;Chen Y;Vandenbark AA;Murphy SJ;Offner H
通讯作者：
Offner H
影响因子：
4.4
作者：
Klehmet, Juliane;Hoffmann, Sarah;Meisel, Andreas
通讯作者：
Meisel, Andreas
影响因子：
3.3
作者：
Vogelgesang, Antje;Dressel, Alexander
通讯作者：
Dressel, Alexander
影响因子：
3.6
作者：
Ebert, LA;Schaerli, P;Moser, B
通讯作者：
Moser, B
影响因子：
64.8
作者：
Champagne, P;Ogg, GS;Pantaleo, G
通讯作者：
Pantaleo, G