Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.
Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.
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DOI：
10.1038/s41467-018-06701-4
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发表时间：
2018-10-10
影响因子：
16.6
通讯作者：
Roe AJ
中科院分区：
文献类型：
--
作者：
Connolly JPR;Slater SL;O'Boyle N;Goldstone RJ;Crepin VF;Ruano-Gallego D;Herzyk P;Smith DGE;Douce GR;Frankel G;Roe AJ
细菌病原体的生态位适应取决于识别来自环境的信号的复杂性并将该信息与对感染至关重要的基因的调节相结合的能力。在这里，我们报告了在其天然鼠类宿主感染期间附着和消除病原体柠檬酸杆菌的转录组。体内病原体基因表达严重偏向于毒力因子库，并且发现其对宿主的反应具有独特的协调性。一致地，我们确定了与毒力调节重叠的代谢途径的宿主特异性诱导。研究发现，重要的 3 型分泌系统和一组相关的不同效应器通过一种独特的机制进行协调调节，该机制涉及微生物群衍生的 1,2-丙二醇的代谢，这决定了有效定植宿主的能力。这项研究提供了关于宿主特异性代谢适应如何作为微调毒力的线索的新见解。小鼠感染啮齿类柠檬酸杆菌是一种常见的附着和消除病原体感染模型。在这里，康诺利等人。分析小鼠感染期间 C. rodentium 的转录组，显示宿主诱导的毒力因子和 1,2-丙二醇代谢的协调上调。
Niche-adaptation of a bacterial pathogen hinges on the ability to recognize the complexity of signals from the environment and integrate that information with the regulation of genes critical for infection. Here we report the transcriptome of the attaching and effacing pathogen Citrobacter rodentium during infection of its natural murine host. Pathogen gene expression in vivo was heavily biased towards the virulence factor repertoire and was found to be co-ordinated uniquely in response to the host. Concordantly, we identified the host-specific induction of a metabolic pathway that overlapped with the regulation of virulence. The essential type 3 secretion system and an associated suite of distinct effectors were found to be modulated co-ordinately through a unique mechanism involving metabolism of microbiota-derived 1,2-propanediol, which dictated the ability to colonize the host effectively. This study provides novel insights into how host-specific metabolic adaptation acts as a cue to fine-tune virulence. Infection of mice with Citrobacter rodentium is a common model of infection with attaching-and-effacing pathogens. Here, Connolly et al. analyse the transcriptome of C. rodentium during mouse infection, showing host-induced coordinated upregulation of virulence factors and 1,2-propanediol metabolism.
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DOI：
10.1038/ismej.2014.242
发表时间：
2015-03-17
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.4
作者：
Carlson-Banning KM;Sperandio V
通讯作者：
Sperandio V
影响因子：
7
作者：
Deng, Wanyin;Yu, Hong B.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett
影响因子：
3.6
作者：
Deng, WY;Vallance, BA;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
3.1
作者：
Ale A;Crepin VF;Collins JW;Constantinou N;Habibzay M;Babtie AC;Frankel G;Stumpf MPH
通讯作者：
Stumpf MPH