Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii.
Transcriptomic and phenotype analysis revealed the role of rpoS in stress resistance and virulence of pathogenic Enterobacter cloacae from Macrobrachium rosenbergii.
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DOI：
10.3389/fmicb.2022.1030955
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发表时间：
2022
影响因子：
5.2
通讯作者：
Zhang, Xiaojun
中科院分区：
文献类型：
--
作者：
Gao, Xiaojian;Qian, Qieqi;Zhu, Yujie;Chen, Zhen;Xu, Jingwen;Xu, Wenjing;Jiang, Qun;Wang, Jun;Zhang, Xiaojun
阴沟肠杆菌广泛分布于水环境中，是近年来被确定为多种水生动物的新病原菌。我们以前的研究表明E. Closophy引起罗氏沼虾的反复感染，表明细菌的高存活能力，并且已知rpoS基因调节许多细菌的应激反应和毒力。在本研究中，E.利用RNAi技术构建了泄殖腔rpoS RNAi菌株，研究了rpoS基因对泄殖腔抗逆性和毒力的调控作用。通过转录组学和表型分析来探索cloprotein。转录组分析结果显示，rpoS-RNAi与野生型菌株共鉴定出488个差异表达基因，其中上调基因30个，下调基因458个，下调基因主要与环境反应、生物膜形成、细菌II型分泌系统、鞭毛蛋白、菌毛蛋白、趋化蛋白等与细菌存活和毒力相关的基因有关。表型变化也表明E.结果表明，Cloacae-rpoS RNAi菌株在环境胁迫（饥饿、盐度、低pH和氧化胁迫）下的生存能力、生物膜形成能力、运动能力、对细胞的粘附能力、致病性和对M.罗森伯托。这些结果表明，rpoS在大肠杆菌的环境应激适应和毒力中发挥着重要的调节作用。clothing.
Enterobacter cloacae is widely distributed in the aquatic environment, and has been determined as a novel pathogen of various aquatic animals recently. Our previous studies have indicated E. cloacae caused repeated infections in Macrobrachium rosenbergii, suggesting a high survival ability of the bacteria, and rpoS gene has been known to regulate stress response and virulence of many bacteria. In this study, the E. cloacae-rpoS RNAi strain was constructed by RNAi technology, and the regulation role of rpoS in stress resistance and virulence of E. cloacae was explored by transcriptomic and phenotype analysis. The transcriptome analysis showed a total of 488 differentially expressed genes (DEGs) were identified between rpoS-RNAi and wild-type strains, including 30 up-regulated genes and 458 down-regulated genes, and these down-regulated DEGs were mainly related to environmental response, biofilm formation, bacterial type II secretory system, flagellin, fimbrillin, and chemotactic protein which associated with bacterial survival and virulence. The phenotype changes also showed the E. cloacae-rpoS RNAi strain exhibited significantly decreasing abilities of survival in environmental stresses (starvation, salinity, low pH, and oxidative stress), biofilm production, movement, adhesion to cells, pathogenicity, and colonization to M. rosenbergii. These results reveal that rpoS plays an important regulatory role in environmental stress adaptation and virulence of E. cloacae.
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影响因子：
4.4
作者：
Hülsmann, A;Rosche, TM;Oliver, JD
通讯作者：
Oliver, JD
影响因子：
28.3
作者：
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通讯作者：
Hadi, Sibte
影响因子：
4.5
作者：
Gao, Xiaojian;Zhang, Honghua;Zhang, Xiaojun
通讯作者：
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影响因子：
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作者：
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通讯作者：
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