Systematic Analysis of Alkaline/Neutral Invertase Genes Reveals the Involvement of Smi-miR399 in Regulation of SmNINV3 and SmNINV4 in Salvia miltiorrhiza
Systematic Analysis of Alkaline/Neutral Invertase Genes Reveals the Involvement of Smi-miR399 in Regulation of SmNINV3 and SmNINV4 in Salvia miltiorrhiza
复制标题
碱性/中性蔗糖酶基因的系统分析揭示了丹参中 Smi-miR399 参与 SmNINV3 和 SmNINV4 的调节
DOI：
10.3390/plants8110490
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发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Lu Shanfa
中科院分区：
文献类型：
--
作者：
Zhou hong;Li Caili;Qiu Xiaoxiao;Lu Shanfa
碱性/中性转化酶（Alkaline/neutral invertases，NINVs）是一类不可逆地催化蔗糖水解为果糖和葡萄糖的酶，在碳水化合物代谢和植物生长发育中起着重要作用。丹参（Salvia miltiorrhiza）是一种具有重要药用价值和经济价值的传统中药植物，目前对丹参中NINV基因的研究尚不全面。通过全基因组预测，确定了9个推定的SmNINV基因，称为SmNINV 1-SmNINV 9。综合分析基因结构、序列特征、保守结构域、保守基序和系统发育树，揭示了SmNINVs的保守性和多样性。所鉴定的SmNINVs在根、茎、叶、花和不同根组织中差异表达。他们也响应干旱，水杨酸，酵母提取物，茉莉酸甲酯治疗。更重要的是，计算预测和实验验证表明，SmNINV 3和SmNINV 4是Smi-miR 399的靶点，Smi-miR 399是一种保守的miRNA，先前显示通过磷酸盐2（PHO 2）的切割影响Pi的摄取和易位。一致地，来自拟南芥、杨、木薯和番茄的43个NINV基因和26个miR 399序列的分析表明，多种AtNINV、PtNINV、MeNINV和SlNINV基因受miR 399调控。这表明miR 399-NINV模块在植物中广泛存在。此外，Smi-miR 399还切割S.丹参，表明NINVs，PHO 2和miR 399网络的复杂性。
Alkaline/neutral invertases (NINVs), which irreversibly catalyze the hydrolysis of sucrose into fructose and glucose, play crucial roles in carbohydrate metabolism and plant development. Comprehensive insights into NINV genes are lacking in Salvia miltiorrhiza, a well-known traditional Chinese medicinal (TCM) plant with significant medicinal and economic value. Through genome-wide prediction, nine putative SmNINV genes, termed SmNINV1-SmNINV9, were identified. Integrated analysis of gene structures, sequence features, conserved domains, conserved motifs and phylogenetic trees revealed the conservation and divergence of SmNINVs. The identified SmNINVs were differentially expressed in roots, stems, leaves, flowers, and different root tissues. They also responded to drought, salicylic acid, yeast extract, and methyl jasmonate treatments. More importantly, computational prediction and experimental validation showed that SmNINV3 and SmNINV4 were targets of Smi-miR399, a conserved miRNA previously shown to affect Pi uptake and translocation through the cleavage of PHOSPHATE2 (PHO2). Consistently, analysis of 43 NINV genes and 26 miR399 sequences from Arabidopsis thaliana, Populus trichocarpa, Manihot esculenta, and Solanum lycopersicum showed that various AtNINV, PtNINV, MeNINV, and SlNINV genes were regulated by miR399. It indicates that the miR399-NINV module exists widely in plants. Furthermore, Smi-miR399 also cleaved SmPHO2 transcripts in S. miltiorrhiza, suggesting the complexity of NINVs, PHO2, and miR399 networks.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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