Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro.
Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro.
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Galectin-1 对体外猪卵巢颗粒细胞黄体酮产生的调节作用。
作者：
H. Walzel;J. Brock;R. Pöhland;J. Vanselow;W. Tomek;F. Schneider;U. Tiemann
半乳糖凝集素-1（gal-1）在猪颗粒细胞裂解物中的免疫印迹检测及其胞浆和膜相关定位促使我们研究其对细胞增殖和调节孕酮合成的影响。外源凝集素刺激无血清培养的猪卵巢颗粒细胞增殖。Gal-1抑制FSH刺激的颗粒细胞孕酮合成。这种抑制作用被强烈减少的disease-inducible竞争对手乳糖在30 mM。对二丁酰-cAMP（DB-cAMP），毛喉素，和炔雌醇酮增强细胞孕酮合成的抑制作用的情况下，表明半乳糖-1干扰FSH刺激的孕酮生产的受体依赖性机制。在FSH刺激的颗粒细胞中，蛋白质印迹分析揭示了半乳糖-1介导的细胞色素P450依赖性胆固醇侧链裂解酶（P450（SCC））的抑制，该酶催化胆固醇转化为双烯醇酮。在30 mM乳糖的存在下，半乳糖-1-减少P450（SCC）的表达被阻止。当FSH刺激的颗粒细胞在gal-1存在下培养时，记录到P450（SCC）和3 β-羟基类固醇脱氢酶/异构酶（3 β-HSD）的mRNA水平显著降低。我们的结论是，半乳糖-1发挥其抑制作用的颗粒细胞的类固醇生成活性的干扰FSH-受体的相互作用，导致反应降低FSH刺激。
The detection of galectin-1 (gal-1) in pig granulosa cell lysates by immunoblotting and its cytosolic as well as membrane-associated localization prompted us to study its effects on cell proliferation and regulation of progesterone synthesis. The lectin stimulated the proliferation of granulosa cells from pig ovaries cultured in serum-free medium. Gal-1 inhibited the FSH-stimulated progesterone synthesis of granulosa cells. This inhibitory effect was strongly reduced by the disaccharidic competitor lactose at 30 mM. The absence of inhibitory effects on dibutyryl-cAMP (db-cAMP), forskolin, and pregnenolone-enhanced cellular progesterone synthesis suggests that gal-1interferes with the receptor-dependent mechanism of FSH-stimulated progesterone production. In FSH-stimulated granulosa cells, western blot analysis revealed the gal-1-mediated suppression of the cytochrome P450-dependent cholesterol side chain cleavage enzyme (P450(SCC)) that catalyzes the conversion of cholesterol to pregnenolone. In the presence of 30 mM lactose, the gal-1-reduced P450(SCC) expression was prevented. Strongly reduced mRNA levels were recorded for P450(SCC) and 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD) when FSH-stimulated granulosa cells were cultured in the presence of gal-1. We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone-receptor interaction resulting in decreased responses to FSH stimulation.
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影响因子：
20.3
作者：
J. Richards
通讯作者：
J. Richards
DOI：
--
发表时间：
1994-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Barondes;D. Cooper;M. Gitt;H. Leffler
通讯作者：
S. Barondes;D. Cooper;M. Gitt;H. Leffler
牛 CYP11A (P-450scc) 基因启动子/调控区的表征。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ahlgren,R;Simpson,ER;Waterman,MR;Lund,J
通讯作者：
Lund,J
Madin-Darby 犬肾细胞顶端分泌胞浆蛋白。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lindstedt,R;Apodaca,G;Barondes,SH;Mostov,KE;Leffler,H
通讯作者：
Leffler,H
卵泡刺激素可增加猪颗粒细胞原代培养物中编码细胞色素 P450 胆固醇侧链裂解酶的信使核糖核酸的浓度。
影响因子：
4.8
作者：
Urban,RJ;Garmey,JC;Shupnik,MA;Veldhuis,JD
通讯作者：
Veldhuis,JD