Validating and troubleshooting ocular in vitro toxicology tests.
Validating and troubleshooting ocular in vitro toxicology tests.
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DOI：
10.1016/j.vascn.2010.01.001
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发表时间：
2010-03
影响因子：
1.9
通讯作者：
Barile FA
中科院分区：
文献类型：
--
作者：
Barile FA
用于测试眼刺激物的体外器官型模型作为替代体内眼测试的方法已经引起足够的兴趣。体外器官型模型声称在隔离系统中维持哺乳动物角膜的短期正常生理和生化功能。在这些试验方法中，通过分别使用浊度计和分光光度计定量测量角膜混浊度和渗透性的变化来评估供试品的损伤。两种测量值均定量用于刺激性分类，以预测供试品的体内眼部刺激潜力。结合这些标准的器官型模型的实例包括：牛角膜混浊和渗透性（BCOP）测定、离体鸡眼（ICE）测试方法和离体兔眼（IRE）测定。第四种方法，鸡卵试验-绒毛尿囊膜（HET-CAM）测定，在评价标准上不同，但通常也包括在这类体外方案中。这些方案中的每一个都作为评估眼部刺激和腐蚀的代表性候选体外方法进行了详细讨论。的方法，协议的细节，应用程序，和他们的验证状态进行了讨论。一个简短的历史观点的发展，原始的体外眼部测试模型也提到。更重要的是，改进和故障排除目前的技术，以目前的模型作为独立的体外工具，眼毒性评估，强调。
In vitro organotypic models for testing ocular irritants have warranted sufficient interest as methods to replace in vivo ocular testing. The in vitro organotypic models claim to maintain short-term normal physiological and biochemical function of the mammalian cornea in an isolated system. In these test methods, damage by the test substance is assessed by quantitative measurements of changes in corneal opacity and permeability using opacitometry and spectrophotometry, respectively. Both measurements are used quantitatively for irritancy classification for prediction of the in vivo ocular irritation potential of a test substance. Examples of organotypic models that incorporate these criteria include: the bovine corneal opacity and permeability (BCOP) assay, the isolated chicken eye (ICE) test method and the isolated rabbit eye (IRE) assay. A fourth method, the hen's egg test-chorioallantoic membrane (HET-CAM) assay, differs in the evaluation criteria but is also normally included among this class of in vitro protocols. Each of these protocols is discussed in detail as representative candidate in vitro methods for assessing ocular irritation and corrosion. The methodologies, protocol details, applications, and their validation status are discussed. A brief historical perspective of the development of original in vitro ocular testing models is also mentioned. More importantly, improvement and troubleshooting the current techniques, in order to present the models as stand-alone in vitro tools for ocular toxicity assessment, is emphasized.
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影响因子：
6.1
作者：
Demirci, F;Paper, DH;Baser, KHC
通讯作者：
Baser, KHC
影响因子：
3.2
作者：
Calabro, Anthony R.;Konsoula, Roula;Barile, Frank A.
通讯作者：
Barile, Frank A.
DOI：
10.3109/15569529609048870
发表时间：
1996-01-01
期刊：
JOURNAL OF TOXICOLOGY-CUTANEOUS AND OCULAR TOXICOLOGY
影响因子：
--
作者：
Casterton, PL;Potts, LF;Klein, BD
通讯作者：
Klein, BD
DOI：
10.1007/s11626-996-0011-0
发表时间：
1998-09-01
影响因子：
2.1
作者：
Barile, FA;Cardona, M
通讯作者：
Cardona, M
影响因子：
3.2
作者：
Cooper, KJ;Earl, LK;Raabe, H
通讯作者：
Raabe, H