Expression of Isl1 during mouse development.
Expression of Isl1 during mouse development.
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DOI：
10.1016/j.gep.2013.07.001
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发表时间：
2013-12
影响因子：
1.2
通讯作者：
Sun, Yunfu
中科院分区：
文献类型：
--
作者：
Zhuang, Shaowei;Zhang, Qingquan;Zhuang, Tao;Evans, Sylvia M.;Liang, Xingqun;Sun, Yunfu
LIM-homeodomain转录因子Isl 1在胚胎发生过程中的细胞增殖、分化和存活中起着重要作用。为了更好地可视化Isl 1表达并提供对Isl 1在发育过程中的作用的了解，我们产生了Isl 1核LacZ（nLacZ）敲入小鼠系，并通过Xgal染色分析了发育过程中Isl 1 nlacZ的表达，并通过Isl 1和β-半乳糖苷酶抗体的共免疫染色比较了Isl 1 nlacZ与内源性Isl 1的表达。结果表明，在发展过程中Isl 1核LacZ的表达模式，重演其内源性蛋白质的表达。与先前的原位和免疫组织化学数据一致，我们观察到Isl 1 nlacZ在多种组织和细胞类型中表达，包括中枢和外周神经系统、神经视网膜、内耳、咽部中胚层和内胚层及其衍生物（颅面结构、胸腺、甲状腺和气管）、心血管系统（心脏流出道、颈动脉、脐血管、窦房结和房间隔）、胃肠系统（口腔上皮、胃、胰腺、肠系膜）和后肢。在某些情况下，Isl 1 nlacZ似乎比Isl 1蛋白更容易检测时，表达水平低，在其他情况下，Isl 1 nlacZ似乎作为一个谱系示踪剂，可能是由于持久的核定位β-半乳糖苷酶。
The LIM-homeodomain transcription factor Isl1 plays essential roles in cell proliferation, differentiation and survival during embryogenesis. To better visualize Isl1 expression and provide insight into the role of Isl1 during development, we generated an Isl1 nuclear LacZ (nLacZ) knockin mouse line and analyzed Isl1nlacZ expression during development by Xgal staining and compared expression of Isl1nlacZ with endogenous Isl1 by coimmunostaining with antibodies to Isl1 and β-galactosidase. Results demonstrated that during development Isl1 nuclear LacZ is expressed in a pattern that recapitulates its endogenous protein expression. Consistent with previous in situ and immunohistochemistry data, we observed Isl1nlacZ expression in multiple tissues and cell types, including the central and peripheral nervous system, neural retina, inner ear, pharyngeal mesoderm and endoderm and their derivatives (craniofacial structures, thymus, thyroid gland and trachea), cardiovascular system (cardiac outflow tract, carotid arteries, umbilical vessels, sinoatrial node and atrial septum), gastrointestinal system (oral epithelium, stomach, pancreas, mesentery) and hindlimb. In some cases, Isl1nlacZ appears to be more readily detectable than Isl1 protein when expression level is low, and in others, Isl1nlacZ appears to act as a lineage tracer, likely owing to perdurance of the nuclear localized beta-galactosidase.
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