Gene expression profiling in subcutaneous, visceral and epigastric adipose tissues of patients with extreme obesity.
Gene expression profiling in subcutaneous, visceral and epigastric adipose tissues of patients with extreme obesity.
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DOI：
10.1038/ijo.2013.152
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发表时间：
2014-03
影响因子：
4.9
通讯作者：
Argyropoulos, G.
中科院分区：
文献类型：
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作者：
Gerhard, G. S.;Styer, A. M.;Strodel, W. E.;Roesch, S. L.;Yavorek, A.;Carey, D. J.;Wood, G. C.;Petrick, A. T.;Gabrielsen, J.;Ibele, A.;Benotti, P.;Rolston, D. D.;Still, C. D.;Argyropoulos, G.
本研究的目的是确定III类严重肥胖个体中SAT、VAT和EAT储存库之间的基因表达差异。人皮下（SAT）和内脏（VAT）脂肪组织表现出不同的基因表达谱。然而，关于另一个近端白色脂肪组织上腹部（EAT）的功能和对代谢的贡献的信息很少。使用从接受开放式Roux-en-Y胃旁路手术的III类严重肥胖患者的脂肪生物标本中获得的RNA，我们使用经真实的时间定量PCR验证的微阵列比较了SAT、VAT和EAT之间的基因表达谱。这三个基因库共有1,907个基因。在这个仓库中，VAT有最多的基因[66]，其次是SAT [23]，然后是EAT [14]。此外，VAT与EAT [65]比SAT [38]共享更多的基因。使用SAT/EAT、VAT/EAT和SAT/VAT的比率进行的进一步分析鉴定了代表皮肤病、炎症、细胞周期和生长、癌症和发育的基因的特定以及重叠的网络和途径。对在脂肪组织发育和功能中发挥作用的基因进行的靶向分析显示，调节激素鸢尾素（FNCD 5）前体的过氧化物酶体增殖物激活受体γ辅激活因子1-α（PGC1-α）在所有三个脂肪库中大量表达，沿着成纤维细胞生长因子（FGF）FGF1、FGF7和FGF10，而FGF19和FGF21检测不到。这些数据表明，EAT与VAT有更多的共同点，表明类似的代谢潜力。人上腹部脂肪库在代谢性疾病中可能发挥重要的功能作用，值得进一步研究。
The goal of the present study was to identify differences in gene expression between SAT, VAT, and EAT depots in Class III severely obese individuals. Human subcutaneous (SAT) and visceral (VAT) adipose tissues exhibit differential gene expression profiles. There is little information, however, about the other proximal white adipose tissue, epigastric (EAT) in terms of its function and contribution to metabolism. Using RNA from adipose biospecimens obtained from Class III severely obese patients undergoing open Roux-en-Y gastric bypass surgery, we compared gene expression profiles between SAT, VAT, and EAT, using microarrays validated by real time quantitative PCR. The three depots were found to share 1,907 genes. VAT had the greatest number of genes [66] expressed exclusively in this depot, followed by SAT [23], and then EAT [14]. Moreover, VAT shared more genes with EAT [65] than with SAT [38]. Further analyses using ratios of SAT/EAT, VAT/EAT, and SAT/VAT, identified specific as well as overlapping networks and pathways of genes representing dermatological diseases, inflammation, cell cycle and growth, cancer, and development. Targeted analysis of genes playing a role in adipose tissue development and function, revealed that Peroxisome proliferator-activated receptor Gamma Coactivator 1-alpha (PGC1-α) that regulates the precursor of the hormone Irisin (FNCD5), were abundantly expressed in all three fat depots, along with fibroblast growth factors (FGF) FGF1, FGF7, and FGF10, whereas, FGF19 and FGF21 were undetectable. These data indicate that EAT has more in common with VAT suggesting similar metabolic potential. The human epigastric adipose depot could play a significant functional role in metabolic diseases and should be further investigated.
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影响因子：
--
作者：
Lillvis JH;Erdman R;Schworer CM;Golden A;Derr K;Gatalica Z;Cox LA;Shen J;Vander Heide RS;Lenk GM;Hlavaty L;Li L;Elmore JR;Franklin DP;Gray JL;Garvin RP;Carey DJ;Lancaster WD;Tromp G;Kuivaniemi H
通讯作者：
Kuivaniemi H
影响因子：
9.8
作者：
KROTKIEWSKI, M;SJOSTROM, L;SMITH, U
通讯作者：
SMITH, U
影响因子：
64.8
作者：
Hart, AW;Baeza, N;Edlund, H
通讯作者：
Edlund, H
影响因子：
4.8
作者：
Billon, Nathalie;Dani, Christian
通讯作者：
Dani, Christian
影响因子：
2.9
作者：
Matzko, Michelle E.;Argyropoulos, George;Gerhard, Glenn S.
通讯作者：
Gerhard, Glenn S.