Cadmium tolerance and phytochelatin content of Arabidopsis seedlings over-expressing the phytochelatin synthase gene AtPCS1.
Cadmium tolerance and phytochelatin content of Arabidopsis seedlings over-expressing the phytochelatin synthase gene AtPCS1.
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DOI：
10.1093/jxb/err228
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发表时间：
2011-11
影响因子：
6.9
通讯作者：
Cardarelli M
中科院分区：
文献类型：
--
作者：
Brunetti P;Zanella L;Proia A;De Paolis A;Falasca G;Altamura MM;Sanità di Toppi L;Costantino P;Cardarelli M
已有研究表明，拟南芥植物螯合素（PC）生物合成基因AtPCS1在烟草中的表达提高了外源谷胱甘肽（GSH）对镉的耐受性。本文对AtPCS1过表达的拟南芥（AtPCSox株系）的镉耐性进行了分析，并比较了拟南芥与烟草的差异。通过对幼苗鲜重、初生根长度和根系解剖结构变化的分析，证明在相对较低的Cd浓度下，AtPCSox品系的Cd耐受性低于野生型，而AtPCS1过表达烟草对Cd的耐受性高于野生型。在较高的Cd浓度下，拟南芥AtPCSox幼苗比野生型更耐受Cd，而烟草AtPCS1幼苗与野生型一样敏感。外源GSH，在烟草中观察到的相反，没有增加AtPCSox线的镉耐受性。在低镉浓度下，野生型拟南芥中的PC含量是烟草中的三倍以上，并且在PC链长度上也发现了实质性差异。这些数据表明，镉的耐受性和依赖外源GSH拟南芥和烟草之间的差异是由于种特异性差异的内源含量的PC和GSH，可能是在不同长度的PC的相对丰度。
Previous studies demonstrated that expression of the Arabidopsis phytochelatin (PC) biosynthetic gene AtPCS1 in Nicotiana tabacum plants increases the Cd tolerance in the presence of exogenous glutathione (GSH). In this paper, the Cd tolerance of Arabidopsis plants over-expressing AtPCS1 (AtPCSox lines) has been analysed and the differences between Arabidopsis and tobacco are shown. Based on the analysis of seedling fresh weight, primary root length, and alterations in root anatomy, evidence is provided that, at relatively low Cd concentrations, the Cd tolerance of AtPCSox lines is lower than the wild type, while AtPCS1 over-expressing tobacco is more tolerant to Cd than the wild type. At higher Cd concentrations, Arabidopsis AtPCSox seedlings are more tolerant to Cd than the wild type, while tobacco AtPCS1 seedlings are as sensitive as the wild type. Exogenous GSH, in contrast to what was observed in tobacco, did not increase the Cd tolerance of AtPCSox lines. The PC content in wild-type Arabidopsis at low Cd concentrations is more than three times higher than in tobacco and substantial differences were also found in the PC chain lengths. These data indicate that the differences in Cd tolerance and in its dependence on exogenous GSH between Arabidopsis and tobacco are due to species-specific differences in the endogenous content of PCs and GSH and may be in the relative abundance of PCs of different length.
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影响因子：
5.1
作者：
Gasic, Ksenija;Korban, Schuyler S.
通讯作者：
Korban, Schuyler S.
影响因子：
4.9
作者：
Durcekova, Katarina;Huttova, Jana;Tamas, Ladislav
通讯作者：
Tamas, Ladislav
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
7.4
作者：
Schützendübel, A;Schwanz, P;Polle, A
通讯作者：
Polle, A