Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle.
Characterization of the First Cultured Representative of "Candidatus Thermofonsia" Clade 2 within Chloroflexi Reveals Its Phototrophic Lifestyle.
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作者：
Zheng R;Cai R;Wang C;Liu R;Sun C
“热刺龙”是绿刺龙门中的一个新种类。宏基因组分析显示“Ca. Thermofonsia”在经典的光养绿鳍藻纲之外拥有光养生物。不幸的是，纯培养物的缺乏限制了对它们潜在的光养作用的进一步了解。在这里，我们报告的成功隔离的“钙。Thermofonsia”代表（Phototrophicus methaneseepsis ZRK 33）从深海冷泉。使用组合的生理学、基因组学和转录组学方法，我们进一步显示了长波长光（例如，红光和红外光）可促进ZRK 33的生长，并上调与光养相关基因的表达。特别是，菌株ZRK 33在实验室和深海条件下都具有典型的光养生活方式。菌株ZRK 33还具有在实验室和深海原位环境中通过3-羟基丙酸自行车固定无机碳的能力，并且组合的自养、光养和异养能力赋予菌株ZRK 33光混合营养的生活方式。值得注意的是，预测的基因与光养广泛存在于27个深海Chloroflexi成员的宏基因组中，强烈表明不同的光养Chloroflexi成员分布在各种未开发的深海生物圈。
“Candidatus Thermofonsia” represents a novel class within the phylum Chloroflexi. Metagenomic analysis reveals “Ca. Thermofonsia” harbors phototrophs outside the classically phototrophic Chloroflexia class. Unfortunately, the paucity of pure cultures limits further insights into their potential phototrophy. Here, we report the successful isolation of a “Ca. Thermofonsia” representative (Phototrophicus methaneseepsis ZRK33) from a deep-sea cold seep. Using combined physiological, genomic, and transcriptomic methods, we further show the long-wavelength light (e.g., red and infrared light) could promote the growth of strain ZRK33 and upregulate the expression of genes associated with phototrophy. In particular, strain ZRK33 has a typical phototrophic lifestyle under both laboratory and deep-sea conditions. Strain ZRK33 also possesses the ability to fix inorganic carbon through the 3-hydroxypropionate bicycle in both laboratory and deep-sea in situ environments, and the combined autotrophic, phototrophic, and heterotrophic capabilities endow strain ZRK33 with a photomixotrophic lifestyle. Notably, the predicted genes associated with phototrophy broadly exist in the metagenomes of 27 deep-sea Chloroflexi members, strongly suggesting diverse phototrophic Chloroflexi members are distributed in various unexplored deep biospheres.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
16.6
作者：
Hamaguchi T;Kawakami K;Shinzawa-Itoh K;Inoue-Kashino N;Itoh S;Ifuku K;Yamashita E;Maeda K;Yonekura K;Kashino Y
通讯作者：
Kashino Y
DOI：
10.1099/13500872-145-7-1743
发表时间：
1999-07-01
期刊：
MICROBIOLOGY-UK
影响因子：
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作者：
Ivanovsky, RN;Fal, YI;Zyakun, AM
通讯作者：
Zyakun, AM
DOI：
10.1099/ijs.0.055855-0
发表时间：
2014-06-01
影响因子：
2.8
作者：
Dodsworth, Jeremy A.;Gevorkian, Jonathan;Hedlund, Brian P.
通讯作者：
Hedlund, Brian P.
DOI：
10.1073/pnas.0503674102
发表时间：
2005-06-28
影响因子：
11.1
作者：
Beatty, JT;Overmann, J;Plumley, FG
通讯作者：
Plumley, FG