The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults.
The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults.
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DOI：
10.3389/fcimb.2013.00041
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发表时间：
2013
影响因子：
5.7
通讯作者：
Drake MJ
中科院分区：
文献类型：
--
作者：
Lewis DA;Brown R;Williams J;White P;Jacobson SK;Marchesi JR;Drake MJ
健康个体的尿液微生物组及其随年龄变化的方式尚未得到表征，可能会影响疾病进程。传统的微生物学方法捕获全谱尿液细菌种类的范围有限。我们通过扩增16S rRNA基因研究了26至90岁健康人群的尿液微生物群，并通过454焦磷酸测序分析了扩增产物。中段尿（MSU）采用“清洁收集”法收集。尿液样本中16S rRNA基因的定量PCR允许细菌载量的相对计数。样品分析表明，女性有一个更异质的混合细菌属相比，男性样品，一般有放线菌门和拟杆菌门的代表性成员。对数据的分析使我们得出结论，当样本按年龄分组时，可能存在“核心”尿液微生物组，年龄组之间的丰度存在波动。该研究还揭示了年龄特异性的属Jonquetella，Parvimonas，Proteiniphilum和Acidofermentans。总之，传统的微生物方法不足以完全鉴定本研究中鉴定的约三分之二的细菌。虽然这项原理验证研究由于样本量而存在局限性，但该样本数据中明显的发现强烈表明，应鼓励对尿液微生物组进行更大规模的研究，并且在特定年龄段鉴定特定属可能与临床疾病的发病机制相关。
The urinary microbiome of healthy individuals and the way it alters with ageing have not been characterized and may influence disease processes. Conventional microbiological methods have limited scope to capture the full spectrum of urinary bacterial species. We studied the urinary microbiota from a population of healthy individuals, ranging from 26 to 90 years of age, by amplification of the 16S rRNA gene, with resulting amplicons analyzed by 454 pyrosequencing. Mid-stream urine (MSU) was collected by the “clean-catch” method. Quantitative PCR of 16S rRNA genes in urine samples, allowed relative enumeration of the bacterial loads. Analysis of the samples indicates that females had a more heterogeneous mix of bacterial genera compared to the male samples and generally had representative members of the phyla Actinobacteria and Bacteroidetes. Analysis of the data leads us to conclude that a “core” urinary microbiome could potentially exist, when samples are grouped by age with fluctuation in abundance between age groups. The study also revealed age-specific genera Jonquetella, Parvimonas, Proteiniphilum, and Saccharofermentans. In conclusion, conventional microbiological methods are inadequate to fully identify around two-thirds of the bacteria identified in this study. Whilst this proof-of-principle study has limitations due to the sample size, the discoveries evident in this sample data are strongly suggestive that a larger study on the urinary microbiome should be encouraged and that the identification of specific genera at particular ages may be relevant to pathogenesis of clinical conditions.
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影响因子：
--
作者：
Biagi, Elena;Candela, Marco;Fairweather-Tait, Susan;Franceschi, Claudio;Brigidi, Patrizia
通讯作者：
Brigidi, Patrizia
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
DOI：
10.1099/ijs.0.02668-0
发表时间：
2003-11-01
影响因子：
2.8
作者：
Parshina, SN;Kleerebezem, R;Stams, AJM
通讯作者：
Stams, AJM
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.