Systemic blockade of dopamine D2-like receptors increases high-voltage spindles in the globus pallidus and motor cortex of freely moving rats.
Systemic blockade of dopamine D2-like receptors increases high-voltage spindles in the globus pallidus and motor cortex of freely moving rats.
复制标题
系统性阻断多巴胺 D2 样受体会增加自由活动大鼠苍白球和运动皮层的高压纺锤体
DOI：
10.1371/journal.pone.0064637
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gao GD
中科院分区：
文献类型：
--
作者：
Yang C;Ge SN;Zhang JR;Chen L;Yan ZQ;Heng LJ;Zhao TZ;Li WX;Jia D;Zhu JL;Gao GD
高压纺锤体（HVSs）已被报道在大鼠皮质-基底神经节网络中自发和广泛地出现。我们前期的研究表明，多巴胺耗竭可以显著增加自由运动大鼠苍白球（GP）和运动皮层HVSs的功率和连贯性。然而，多巴胺是否通过作用于多巴胺d1样受体或d2样受体来调节HVS活性尚不清楚。我们采用局部场电位和皮质电图方法同时记录自由运动大鼠在全身给予多巴胺受体拮抗剂或生理盐水后GP和初级运动皮层（M1）的振荡活动。结果显示，多巴胺d2样受体拮抗剂雷氯pride和氟哌啶醇显著增加HVS的数量和持续时间，以及与GP和M1皮层HVS活性相关的相对功率。多巴胺d2样受体拮抗剂也显著增加GP和M1皮质区HVS活性的相干值。相反，选择性多巴胺d1样受体拮抗剂SCH23390对hvs的数量、持续时间、相对强度以及M1和GP之间的hvs相关一致性没有显著影响。综上所述，多巴胺d2样受体而非d1样受体参与了HVS的调控。这支持了多巴胺d2样受体在HVSs调节中的重要作用。纹状体上的siRNA敲除实验证实了我们的结论。
High-voltage spindles (HVSs) have been reported to appear spontaneously and widely in the cortical–basal ganglia networks of rats. Our previous study showed that dopamine depletion can significantly increase the power and coherence of HVSs in the globus pallidus (GP) and motor cortex of freely moving rats. However, it is unclear whether dopamine regulates HVS activity by acting on dopamine D1-like receptors or D2-like receptors. We employed local-field potential and electrocorticogram methods to simultaneously record the oscillatory activities in the GP and primary motor cortex (M1) in freely moving rats following systemic administration of dopamine receptor antagonists or saline. The results showed that the dopamine D2-like receptor antagonists, raclopride and haloperidol, significantly increased the number and duration of HVSs, and the relative power associated with HVS activity in the GP and M1 cortex. Coherence values for HVS activity between the GP and M1 cortex area were also significantly increased by dopamine D2-like receptor antagonists. On the contrary, the selective dopamine D1-like receptor antagonist, SCH23390, had no significant effect on the number, duration, or relative power of HVSs, or HVS-related coherence between M1 and GP. In conclusion, dopamine D2-like receptors, but not D1-like receptors, were involved in HVS regulation. This supports the important role of dopamine D2-like receptors in the regulation of HVSs. An siRNA knock-down experiment on the striatum confirmed our conclusion.
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影响因子：
82.9
作者：
Hommel, JD;Sears, RM;DiLeone, RJ
通讯作者：
DiLeone, RJ
影响因子：
2.5
作者：
Dejean, Cyril;Gross, Christian E.;Boraud, Thomas
通讯作者：
Boraud, Thomas
影响因子：
3.4
作者：
BUZSAKI, G;KENNEDY, B;ZIEGLER, M
通讯作者：
ZIEGLER, M
影响因子：
3.3
作者：
BUZSAKI, G;LASZLOVSZKY, I;VADASZ, C
通讯作者：
VADASZ, C
影响因子：
2.5
作者：
Fontanini, A;Katz, DB
通讯作者：
Katz, DB