Response of Saccharomyces cerevisiae to the stimulation of lipopolysaccharide.
Response of Saccharomyces cerevisiae to the stimulation of lipopolysaccharide.
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酿酒酵母对脂多糖刺激的反应
DOI：
10.1371/journal.pone.0104428
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Shen L;Li Y;Jiang L;Wang X
脂多糖被称为内毒素，可以通过Toll样受体4和骨髓分化蛋白2的复合物刺激有效的宿主免疫反应；但它对研究真核生物的模式生物酿酒酵母的影响尚不清楚。在本研究中，我们发现脂多糖处理的酿酒酵母细胞可以被亚甲蓝染色，但不会死亡。脂多糖处理的酿酒酵母细胞的转录谱显示，5745 个基因受到调节：2491 个基因上调，3254 个基因下调。在Gene Ontology上分析脂多糖处理的酿酒酵母细胞中显着调控的基因（460个上调基因和135个下调基因），并用于建立物理蛋白质-蛋白质相互作用网络和蛋白质磷酸化网络。基于这些分析，脂多糖处理的酿酒酵母细胞中的大多数调控基因与细胞壁、细胞膜、过氧化物酶体和线粒体有关。进一步的实验表明，脂多糖刺激引起酿酒酵母细胞中磷脂酰丝氨酸的暴露和线粒体膜电位的增加，但细胞内活性氧水平和元半胱天冬酶激活并未增加。这项研究表明，脂多糖刺激会引起酿酒酵母细胞的显着变化，结果将有助于了解真核细胞对脂多糖刺激的反应。
Lipopolysaccharide, known as endotoxin, can stimulate potent host immune responses through the complex of Toll-like-receptor 4 and myeloid differentiation protein 2; but its influence on Saccharomyces cerevisiae, a model organism for studying eukaryotes, is not clear. In this study, we found that lipopolysaccharide-treated S. cerevisiae cells could be stained by methylene blue, but did not die. Transcriptional profiling of the lipopolysaccharide-treated S. cerevisiae cells showed that 5745 genes were modulated: 2491 genes up-regulated and 3254 genes down-regulated. Significantly regulated genes (460 up-regulated genes and 135 down-regulated genes) in lipopolysaccharide-treated S. cerevisiae cells were analyzed on Gene Ontology, and used to establish physical protein-protein interaction network and protein phosphorylation network. Based on these analyses, most of the regulated genes in lipopolysaccharide-treated S. cerevisiae cells were related to cell wall, membrane, peroxisome and mitochondrion. Further experiments demonstrated that lipopolysaccharide stimulation caused the exposure of phosphatidylserine and the increase of mitochondrial membrane potential in S. cerevisiae cells, but levels of intracellular reactive oxygen species and metacaspase activation were not increased. This study demonstrated that lipopolysaccharide stimulation causes significant changes in S. cerevisiae cells, and the results would contribute to understand the response of eukaryotic cells to lipopolysaccharide stimulation.
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影响因子：
3.9
作者：
Jakubowski, W;Bartosz, G
通讯作者：
Bartosz, G
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通讯作者：
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通讯作者：
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