Electroacupuncture alleviates perioperative hypothalamus-pituitary-adrenal axis dysfunction via circRNA-miRNA-mRNA networks.
Electroacupuncture alleviates perioperative hypothalamus-pituitary-adrenal axis dysfunction via circRNA-miRNA-mRNA networks.
复制标题
电针通过circRNA-miRNA-mRNA网络改善围手术期下丘脑-垂体-肾上腺轴功能障碍
DOI：
10.3389/fnmol.2023.1115569
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发表时间：
2023
影响因子：
4.8
通讯作者：
Tian, Zhanzhuang
中科院分区：
文献类型：
--
作者：
Wang, Yu;Hu, Wei;Han, Jing;Zheng, Jiayuan;Jiang, Ning;Feng, Yi;Tian, Zhanzhuang
关键词：
电针（EA）长期以来一直用于缓解手术引起的下丘脑-垂体-肾上腺轴功能障碍。然而，其在大脑中的下游基因目标仍不清楚。本研究的目的是基于 RNA 测序技术 (RNA-seq) 阐明 EA 的潜在靶点。将大鼠分为正常对照（NC）、肝切除手术（HT）、HT + EA 和 HT + sham EA 组，然后对下丘脑和杏仁核的两个代表性核进行 RNA-seq。加权基因共表达网络分析和基因集富集分析确定了与下丘脑神经内分泌递质和神经重塑相关的六个基因模块。此外，circRNA-miRNA-mRNA相互作用网络揭示了EA相关的候选miRNA和circRNA，其中阿片受体mu 1可能是EA特异性靶标，并显示出通过竞争性内源RNA的调节。我们确定了电针影响 HPA 轴功能障碍的神经内分泌 circRNA-miRNA-mRNA 网络，从而为电针治疗提供了潜在靶点和未来研究方向。
Electroacupuncture (EA) has long been used to alleviate surgery-induced hypothalamic–pituitary–adrenal axis dysfunction. However, its downstream gene targets in the brain remain unclear. The aim of the present study was to clarify the potential targets of EA based on RNA sequencing techniques (RNA-seq). Rats were divided into normal control (NC), hepatectomy surgery (HT), HT + EA, and HT + sham EA groups followed by RNA-seq of two representative nuclei in the hypothalamus and amygdala. Weighted Gene Co-expression Network Analysis and Gene Set Enrichment Analysis identified six gene modules associated with neuroendocrine transmitters and neural remodeling in the hypothalamus. Furthermore, circRNA-miRNA-mRNA interaction networks revealed EA-related candidate miRNAs and circRNAs, of which opioid receptor mu 1 might be an EA-specific target, and showed regulation by competing endogenous RNA. We identified the neuroendocrine circRNA-miRNA-mRNA networks through which EA has an effect on HPA axis dysfunction, thus providing potential targets and future research directions for EA treatment.
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影响因子：
4.9
作者：
Han X;Gao Y;Yin X;Zhang Z;Lao L;Chen Q;Xu S
通讯作者：
Xu S
影响因子：
3.6
作者：
He, Takeshi;Shum, Rebecca;Pan, Ying-Xian
通讯作者：
Pan, Ying-Xian
影响因子：
25
作者：
Daviu, Nuria;Fuzesi, Tamas;Bains, Jaideep S.
通讯作者：
Bains, Jaideep S.
影响因子：
5.1
作者：
Hepsen, Sema;Sencar, Erkam;Cakal, Erman
通讯作者：
Cakal, Erman
DOI：
10.1155/2018/8041820
发表时间：
2018
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Jiang SW;Lin YW;Hsieh CL
通讯作者：
Hsieh CL