Increased mechanosensitivity of cells cultured on nanotopographies.
Increased mechanosensitivity of cells cultured on nanotopographies.
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DOI：
10.1016/j.jbiomech.2010.07.015
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发表时间：
2010-11-16
影响因子：
2.4
通讯作者：
Donahue, Henry J.
中科院分区：
文献类型：
--
作者：
Salvi, Joshua D.;Lim, Jung Yul;Donahue, Henry J.
增强细胞的机械敏感性被认为是成功的肌肉骨骼组织工程的新工具。我们验证了这样一种假设，即相对于平面而言，人骨髓间充质干细胞(HMSCs)在纳米图形基质上的机械敏感性增强。将hMSCs培养在聚合物分离的、随机分布的具有不同岛高的纳米岛表面上，并量化了细胞内钙离子浓度[Ca~(2+)]i对流体诱导的剪应力的响应。在特定尺度的纳米拓扑图上培养的干细胞显示出对流体流动的更大的细胞内钙反应。培养在10-20 nm高的纳米岛上的hMSCs的细胞对钙的反应率高于培养在平坦对照上的细胞，并且相对于培养在其他纳米岛(45-80 nm高的纳米岛)上的细胞表现出更大的平均[Ca~(2+)]i升高。由于[Ca~(2+)]i是hMSCs下游信号以及增殖和分化的重要调节因子，这一观察结果表明特定尺度的纳米结构为促进干细胞的机械转导活性提供了最佳的环境。机械信号和底物纳米拓扑学可以协同调节细胞行为，这在再生医学方案的发展中具有重要意义。
Enhancing cellular mechanosensitivity is recognized as a novel tool for successful musculoskeletal tissue engineering. We examined the hypothesis that mechanosensitivity of human mesenchymal stem cells (hMSCs) is enhanced on nanotopographic substrates relative to flat surfaces. hMSCs were cultured on polymer-demixed, randomly distributed nanoisland surfaces with varying island heights and changes in intracellular calcium concentration, [Ca2+]i, in response to fluid flow induced shear stress were quantifide. Stem cells cultured on specific scale nanotopographies displayed greater intracellular calcium responses to fluid flow. hMSCs cultured on 10-20 nm high nanoislands displayed a greater percentage of cells responding in calcium relative to cells cultured on flat control, and showed greater average [Ca2+]i increase relative to cells cultured on other nanoislands (45-80 nm high nanoislands). As [Ca2+]i is an important regulator of downstream signaling, as well as proliferation and differentiation of hMSCs, this observation suggests that specific scale nanotopographies provide an optimal milieu for promoting stem cell mechanotransduction activity. That mechanical signals and substrate nanotopography may synergistically regulate cell behavior is of significant interest in the development of regenerative medicine protocols.
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影响因子：
2.8
作者：
Riddle, Ryan C.;Hippe, Karen R.;Donahue, Henry J.
通讯作者：
Donahue, Henry J.
影响因子：
2.4
作者：
WEINBAUM, S;COWIN, SC;ZENG, Y
通讯作者：
ZENG, Y
DOI：
10.1016/j.ijom.2007.12.008
发表时间：
2008-05-01
影响因子：
2.4
作者：
Qi, M. -C.;Hu, J.;Han, L. -C.
通讯作者：
Han, L. -C.
影响因子：
6.2
作者：
Lim, JY;Hansen, JC;Donahue, HJ
通讯作者：
Donahue, HJ
影响因子：
3.9
作者：
Lim, JY;Hansen, JC;Donahue, HJ
通讯作者：
Donahue, HJ