Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet.
Microbiota and Metabolomic Patterns in the Breast Milk of Subjects with Celiac Disease on a Gluten-Free Diet.
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乳糜泻无麸质饮食受试者母乳中的微生物群和代谢组学模式
DOI：
10.3390/nu13072243
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发表时间：
2021-06-29
期刊：
影响因子：
5.9
通讯作者：
Leonard MM
中科院分区：
文献类型：
--
作者：
Olshan KL;Zomorrodi AR;Pujolassos M;Troisi J;Khan N;Fanelli B;Kenyon V;Fasano A;Leonard MM
当暴露在饮食面筋中时，肠道微生物群可能会引发具有遗传倾向的人的乳糜泻(CD)。研究表明，婴儿期的营养对肠道微生物群的植入至关重要。到目前为止，很少有研究关注无麸质饮食中有CD病史的受试者的母乳成分。在这里，我们利用多组学方法和鸟枪式元基因组学相结合的方法，分析了36名受试者、20名CD患者和16名健康对照的母乳微生物组和代谢组谱。这些分析确定了细菌和病毒物种/菌株和功能途径的显著差异，但在代谢物丰度方面没有差异。具体地说，在接受无麸质饮食的CD受试者中，发现了三种丰度增加的细菌菌株，其中一种(粘液玫瑰属)以前曾被认为与自身免疫疾病有关。我们还确定了五种途径，在无面筋饮食的镉受试者中，镉的丰度增加。此外，我们还发现在健康对照组中有四种细菌和两种病毒物种/菌株的丰度增加。总体而言，在我们的分析中观察到的细菌和病毒物种/菌株以及功能途径的差异可能会影响新生儿的微生物组植入，这可能会影响他们未来的临床结果。
The intestinal microbiome may trigger celiac disease (CD) in individuals with a genetic disposition when exposed to dietary gluten. Research demonstrates that nutrition during infancy is crucial to the intestinal microbiome engraftment. Very few studies to date have focused on the breast milk composition of subjects with a history of CD on a gluten-free diet. Here, we utilize a multi-omics approach with shotgun metagenomics to analyze the breast milk microbiome integrated with metabolome profiling of 36 subjects, 20 with CD on a gluten-free diet and 16 healthy controls. These analyses identified significant differences in bacterial and viral species/strains and functional pathways but no difference in metabolite abundance. Specifically, three bacterial strains with increased abundance were identified in subjects with CD on a gluten-free diet of which one (Rothia mucilaginosa) has been previously linked to autoimmune conditions. We also identified five pathways with increased abundance in subjects with CD on a gluten-free diet. We additionally found four bacterial and two viral species/strains with increased abundance in healthy controls. Overall, the differences observed in bacterial and viral species/strains and in functional pathways observed in our analysis may influence microbiome engraftment in neonates, which may impact their future clinical outcomes.
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影响因子：
5.2
作者：
Biagi E;Aceti A;Quercia S;Beghetti I;Rampelli S;Turroni S;Soverini M;Zambrini AV;Faldella G;Candela M;Corvaglia L;Brigidi P
通讯作者：
Brigidi P
DOI：
10.1016/j.trsl.2016.09.003
发表时间：
2017-01
期刊：
Translational research : the journal of laboratory and clinical medicine
影响因子：
--
作者：
Blázquez AB;Berin MC
通讯作者：
Berin MC
影响因子：
3.6
作者：
Jost, Ted;Lacroix, Christophe;Chassard, Christophe
通讯作者：
Chassard, Christophe
影响因子：
3.7
作者：
Hasan NA;Young BA;Minard-Smith AT;Saeed K;Li H;Heizer EM;McMillan NJ;Isom R;Abdullah AS;Bornman DM;Faith SA;Choi SY;Dickens ML;Cebula TA;Colwell RR
通讯作者：
Colwell RR
影响因子：
158.5
作者：
Lionetti, Elena;Castellaneta, Stefania;Catassi, Carlo
通讯作者：
Catassi, Carlo