Microbial Community Dynamics in Mother's Milk and Infant's Mouth and Gut in Moderately Preterm Infants.
Microbial Community Dynamics in Mother's Milk and Infant's Mouth and Gut in Moderately Preterm Infants.
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DOI：
10.3389/fmicb.2018.02512
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发表时间：
2018
影响因子：
5.2
通讯作者：
Brigidi P
中科院分区：
文献类型：
--
作者：
Biagi E;Aceti A;Quercia S;Beghetti I;Rampelli S;Turroni S;Soverini M;Zambrini AV;Faldella G;Candela M;Corvaglia L;Brigidi P
母乳是早产儿营养的最佳来源，因为它能促进免疫防御和胃肠道功能，预防坏死性小肠结肠炎，改善长期临床结果，并被认为能驱动肠道微生物群组装。早产儿在出生时通常不会直接从母乳中获得母乳，因为活跃的吮吸和吮吸，吞咽和呼吸之间的协调直到胎龄32-34周才发展，但随着年龄的增长，实际母乳喂养通常是可能的。在这里，我们招募了中度早产儿（胎龄32-34周），通过16 S rRNA测序纵向表征母乳和婴儿的肠道和口腔微生物组，直到出生后200多天。这个特殊的人群提供了一个机会，可以解开母乳喂养本身与实际母乳喂养在婴儿微生物组发育中的差异贡献，这两者都被认为是短期和长期婴儿健康状况的关键因素。在这个队列中，乳汁微生物组的组成似乎随着婴儿对母亲乳房的闭锁而改变，转向由典型口腔微生物主导的更多样化的微生物群落，即，链球菌和罗氏菌。即使本研究中的所有婴儿都是母乳喂养的，健康、足月、纯母乳喂养婴儿的典型特征，即，在实际母乳喂养开始后采集样本中，分别在粪便和口腔样本中检测到较高百分比的双歧杆菌和较低丰度的假单胞菌。这些发现强调了不仅鼓励母乳喂养，而且鼓励早产儿早期开始实际母乳喂养的重要性，因为婴儿与母亲乳房的接触可能构成一个独立因素，有助于促进婴儿肠道微生物组的健康。
Mother’s own milk represents the optimal source for preterm infant nutrition, as it promotes immune defenses and gastrointestinal function, protects against necrotizing enterocolitis, improves long-term clinical outcome and is hypothesized to drive gut microbiota assembly. Preterm infants at birth usually do not receive their mother’s milk directly from the breast, because active suckling and coordination between suckling, swallowing and breathing do not develop until 32–34 weeks gestational age, but actual breastfeeding is usually possible as they grow older. Here, we enrolled moderately preterm infants (gestational age 32–34 weeks) to longitudinally characterize mothers’ milk and infants’ gut and oral microbiomes, up to more than 200 days after birth, through 16S rRNA sequencing. This peculiar population offers the chance to disentangle the differential contribution of human milk feeding per se vs. actual breastfeeding in the development of infant microbiomes, that have both been acknowledged as crucial contributors to short and long-term infant health status. In this cohort, the milk microbiome composition seemed to change following the infant’s latching to the mother’s breast, shifting toward a more diverse microbial community dominated by typical oral microbes, i.e., Streptococcus and Rothia. Even if all infants in the present study were fed human milk, features typical of healthy, full term, exclusively breastfed infants, i.e., high percentages of Bifidobacterium and low abundances of Pseudomonas in fecal and oral samples, respectively, were detected in samples taken after actual breastfeeding started. These findings underline the importance of encouraging not only human milk feeding, but also an early start of actual breastfeeding in preterm infants, since the infant’s latching to the mother’s breast might constitute an independent factor helping the health-promoting assembly of the infant gut microbiome.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.7
作者：
Hu J;Nomura Y;Bashir A;Fernandez-Hernandez H;Itzkowitz S;Pei Z;Stone J;Loudon H;Peter I
通讯作者：
Peter I
影响因子：
5.2
作者：
Dobbler PT;Procianoy RS;Mai V;Silveira RC;Corso AL;Rojas BS;Roesch LFW
通讯作者：
Roesch LFW
影响因子：
5.4
作者：
Jeurink, P. V.;van Bergenhenegouwen, J.;Martin, R.
通讯作者：
Martin, R.
影响因子：
2.5
作者：
Cong X;Judge M;Xu W;Diallo A;Janton S;Brownell EA;Maas K;Graf J
通讯作者：
Graf J