Panorama of ancient metazoan macromolecular complexes.
Panorama of ancient metazoan macromolecular complexes.
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DOI：
10.1038/nature14877
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发表时间：
2015-09-17
期刊：
影响因子：
64.8
通讯作者：
Emili, Andrew
中科院分区：
文献类型：
--
作者：
Wan, Cuihong;Borgeson, Blake;Phanse, Sadhna;Tu, Fan;Drew, Kevin;Clark, Greg;Xiong, Xuejian;Kagan, Olga;Kwan, Julian;Bezginov, Alexandr;Chessman, Kyle;Pal, Swati;Cromar, Graham;Papoulas, Ophelia;Ni, Zuyao;Boutz, Daniel R.;Stoilova, Snejana;Havugimana, Pierre C.;Guo, Xinghua;Malty, Ramy H.;Sarov, Mihail;Greenblatt, Jack;Babu, Mohan;Derry, W. Brent;Tillier, Elisabeth R.;Wallingford, John B.;Parkinson, John;Marcotte, Edward M.;Emili, Andrew
大分子复合物对保守的生物过程至关重要，但它们在动物中的普遍程度尚不清楚。通过将广泛的生化分离与定量质谱相结合，我们直接检测了不同后生动物模型中可溶性多蛋白复合物的组成。使用综合方法，我们生成了一份保护图草案，其中包括100万个假设的高置信度共复杂相互作用，这些相互作用包含了所有现存动物中广泛存在的功能模块。聚类揭示了保护的范围，从可能服务于细胞内护角色至少10亿年的古代真核生物组合，积累了当代成分的祖先复合物，以及与多细胞生物相关的罕见后生动物创新。我们通过在进化距离较远的物种中独立的共分离实验、亲和纯化和功能分析验证了这些预测。这些重构相互作用组的全面性、中心性和模块化反映了它们对动物细胞系统的基本机制意义和适应价值。
Macromolecular complexes are essential to conserved biological processes, but their prevalence across animals is unclear. By combining extensive biochemical fractionation with quantitative mass spectrometry, we directly examined the composition of soluble multiprotein complexes among diverse metazoan models. Using an integrative approach, we then generated a draft conservation map consisting of >1 million putative high-confidence co-complex interactions for species with fully sequenced genomes that encompasses functional modules present broadly across all extant animals. Clustering revealed a spectrum of conservation, ranging from ancient Eukaryal assemblies likely serving cellular housekeeping roles for at least 1 billion years, ancestral complexes that have accrued contemporary components, and rarer metazoan innovations linked to multicellularity. We validated these projections by independent co-fractionation experiments in evolutionarily distant species, by affinity-purification and by functional analyses. The comprehensiveness, centrality and modularity of these reconstructed interactomes reflect their fundamental mechanistic significance and adaptive value to animal cell systems.
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影响因子：
64.8
作者：
Kim, Min-Sik;Pinto, Sneha M.;Getnet, Derese;Nirujogi, Raja Sekhar;Manda, Srikanth S.;Chaerkady, Raghothama;Madugundu, Anil K.;Kelkar, Dhanashree S.;Isserlin, Ruth;Jain, Shobhit;Thomas, Joji K.;Muthusamy, Babylakshmi;Leal-Rojas, Pamela;Kumar, Praveen;Sahasrabuddhe, Nandini A.;Balakrishnan, Lavanya;Advani, Jayshree;George, Bijesh;Renuse, Santosh;Selvan, Lakshmi Dhevi N.;Patil, Arun H.;Nanjappa, Vishalakshi;Radhakrishnan, Aneesha;Prasad, Samarjeet;Subbannayya, Tejaswini;Raju, Rajesh;Kumar, Manish;Sreenivasamurthy, Sreelakshmi K.;Marimuthu, Arivusudar;Sathe, Gajanan J.;Chavan, Sandip;Datta, Keshava K.;Subbannayya, Yashwanth;Sahu, Apeksha;Yelamanchi, Soujanya D.;Jayaram, Savita;Rajagopalan, Pavithra;Sharma, Jyoti;Murthy, Krishna R.;Syed, Nazia;Goel, Renu;Khan, Aafaque A.;Ahmad, Sartaj;Dey, Gourav;Mudgal, Keshav;Chatterjee, Aditi;Huang, Tai-Chung;Zhong, Jun;Wu, Xinyan;Shaw, Patrick G.;Freed, Donald;Zahari, Muhammad S.;Mukherjee, Kanchan K.;Shankar, Subramanian;Mahadevan, Anita;Lam, Henry;Mitchell, Christopher J.;Shankar, Susarla Krishna;Satishchandra, Parthasarathy;Schroeder, John T.;Sirdeshmukh, Ravi;Maitra, Anirban;Leach, Steven D.;Drake, Charles G.;Halushka, Marc K.;Prasad, T. S. Keshava;Hruban, Ralph H.;Kerr, Candace L.;Bader, Gary D.;Iacobuzio-Donahue, Christine A.;Gowda, Harsha;Pandey, Akhilesh
通讯作者：
Pandey, Akhilesh
影响因子：
12.3
作者：
Hart GT;Ramani AK;Marcotte EM
通讯作者：
Marcotte EM
影响因子：
4.1
作者：
de Bie, Prim;van de Sluis, Bart;Klomp, Leo W. J.
通讯作者：
Klomp, Leo W. J.
影响因子：
14.9
作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
通讯作者：
Jensen LJ
影响因子：
64.8
作者：
Hartwell, LH;Hopfield, JJ;Murray, AW
通讯作者：
Murray, AW