Application of the CometChip platform to assess DNA damage in field-collected blood samples from turtles.
Application of the CometChip platform to assess DNA damage in field-collected blood samples from turtles.
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DOI：
10.1002/em.22183
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发表时间：
2018-05
影响因子：
2.8
通讯作者：
Sobol RW
中科院分区：
文献类型：
--
作者：
Sykora P;Chiari Y;Heaton A;Moreno N;Glaberman S;Sobol RW
DNA损伤与基因组不稳定性和细胞和生物体内平衡的逐步破坏有关，导致疾病的发生和寿命的缩短。内源性DNA损伤包括碱基脱氨基、碱基水解、碱基烷基化和代谢诱导的氧化损伤，可导致单链和双链DNA断裂。或者，暴露于环境污染物、辐射或紫外线也会导致外源性DNA损伤。我们以前验证了一种新的，高通量的方法来测量培养的哺乳动物细胞中的DNA损伤水平。这种新的CometChip平台建立在经典的单细胞凝胶电泳或彗星方法学的基础上，广泛用于环境毒理学和分子生物学。我们询问CometChip平台是否可用于测量来自环境实地研究的样本中的DNA损伤。为此，我们确定可以在CometChip平台中成功评估来自多种海龟的有核红细胞，以量化DNA损伤水平。总的来说，我们比较了两个物种的40只动物的DNA损伤水平：箱龟（Terrapene卡罗莱纳）和红耳滑块（Trachemys scripta elegans）。内源性DNA损伤水平在两个物种之间是相同的，但我们确实发现了一些与性别相关的差异和DNA损伤积累的变化。基于这些结果，我们确认CometChip平台可以快速准确地测量大量样品中的DNA损伤，特别适用于使用现场收集的样品进行环境研究。
DNA damage has been linked to genomic instability and the progressive breakdown of cellular and organismal homeostasis, leading to the onset of disease and reduced longevity. Insults to DNA from endogenous sources include base deamination, base hydrolysis, base alkylation and metabolism-induced oxidative damage that can lead to single-strand and double-strand DNA breaks. Alternatively, exposure to environmental pollutants, radiation or ultra-violet light, can also contribute to exogenously derived DNA damage. We previously validated a novel, high through-put approach to measure levels of DNA damage in cultured mammalian cells. This new CometChip Platform builds on the classical single cell gel electrophoresis or comet methodology used extensively in environmental toxicology and molecular biology. We asked whether the CometChip Platform could be used to measure DNA damage in samples derived from environmental field studies. To this end, we determined that nucleated erythrocytes from multiple species of turtle could be successfully evaluated in the CometChip Platform to quantify levels of DNA damage. In total, we compared levels of DNA damage in 40 animals from two species: the box turtle (Terrapene carolina) and the red-eared slider (Trachemys scripta elegans). Endogenous levels of DNA damage were identical between the two species, yet we did discover some sex-linked differences and changes in DNA damage accumulation. Based on these results, we confirm that the CometChip Platform allows for the measurement of DNA damage in a large number of samples quickly and accurately, and is particularly adaptable to environmental studies using field-collected samples.
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DOI：
10.1038/nrc2607
发表时间：
2009-05
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/j.mrrev.2008.03.001
发表时间：
2009-01-01
期刊：
Mutation research
影响因子：
--
作者：
Frenzilli, G;Nigro, M;Lyons, B P
通讯作者：
Lyons, B P
影响因子：
2.7
作者：
Jackson, Petra;Pedersen, Lourdes M.;Wallin, Hakan
通讯作者：
Wallin, Hakan
DOI：
10.1016/j.cbpc.2009.11.006
发表时间：
2010-04-01
影响因子：
3.9
作者：
Bonisoli-Alquati, Andrea;Voris, Andrew;Wyatt, Michael D.
通讯作者：
Wyatt, Michael D.
影响因子：
14.8
作者：
Olive, Peggy L.;Banath, Judit P.
通讯作者：
Banath, Judit P.