Isolation and characterization of a lycopene ε-cyclase gene of Chlorella (Chromochloris) zofingiensis. Regulation of the carotenogenic pathway by nitrogen and light.
Isolation and characterization of a lycopene ε-cyclase gene of Chlorella (Chromochloris) zofingiensis. Regulation of the carotenogenic pathway by nitrogen and light.
复制标题
小球藻（Chromochloris）Zofingiensis的番茄红素ε-周期酶基因的分离和表征。通过氮和光调节类胡萝卜素的途径。
DOI：
10.3390/md10092069
复制
发表时间：
2012-09
期刊：
影响因子：
5.4
通讯作者：
Vargas MA
中科院分区：
文献类型：
--
作者：
Cordero BF;Couso I;Leon R;Rodriguez H;Vargas MA
从绿色小球藻（Chlorella（Chromochloris）zofingiensis，Czlcy-e）中分离番茄红素ε-环化酶基因，并对其进行功能分析。该基因参与类胡萝卜素α-胡萝卜素和叶黄素的形成。Czlcy-e基因编码654个氨基酸的多肽。在C. zofingiensis在大肠杆菌中通过异源互补进行的功能分析表明，该蛋白能够将番茄红素转化为δ-胡萝卜素。此外，还研究了光和氮对胡萝卜素合成途径的调控。zofingiensis与低光照条件相比，高光照胁迫没有增加番茄红素β-环化酶基因（lcy-b）和番茄红素ε-环化酶基因（lcy-e）的mRNA水平，但增加了psy、pds、chyB和bkt基因的转录水平，但触发了次生类胡萝卜素虾青素、玉米黄质和玉米黄质的合成，降低了初级类胡萝卜素α-胡萝卜素、叶黄素、紫黄质和β-胡萝卜素。氮饥饿本身增强了所有基因的mRNA水平，除了lcy-e和pds，但没有触发合成的虾青素，玉米黄质也没有。与单独的强光胁迫相比，强光和氮饥饿联合胁迫显著提高了类胡萝卜素的积累和bkt基因的转录水平。
The isolation and characterization of the lycopene ε-cyclase gene from the green microalga Chlorella (Chromochloris) zofingiensis (Czlcy-e) was performed. This gene is involved in the formation of the carotenoids α-carotene and lutein. Czlcy-e gene encoded a polypeptide of 654 amino acids. A single copy of Czlcy-e was found in C. zofingiensis. Functional analysis by heterologous complementation in Escherichia coli showed the ability of this protein to convert lycopene to δ-carotene. In addition, the regulation of the carotenogenic pathway by light and nitrogen was also studied in C. zofingiensis. High irradiance stress did not increase mRNA levels of neither lycopene β-cyclase gene (lcy-b) nor lycopene ε-cyclase gene (lcy-e) as compared with low irradiance conditions, whereas the transcript levels of psy, pds, chyB and bkt genes were enhanced, nevertheless triggering the synthesis of the secondary carotenoids astaxanthin, canthaxanthin and zeaxanthin and decreasing the levels of the primary carotenoids α-carotene, lutein, violaxanthin and β-carotene. Nitrogen starvation per se enhanced mRNA levels of all genes considered, except lcy-e and pds, but did not trigger the synthesis of astaxanthin, canthaxanthin nor zeaxanthin. The combined effect of both high light and nitrogen starvation stresses enhanced significantly the accumulation of these carotenoids as well as the transcript levels of bkt gene, as compared with the effect of only high irradiance stress.
登录
查看更多内容
DOI：
10.1016/0005-2728(74)90063-2
发表时间：
1974-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ARNON, DI;MCSWAIN, BD;WADA, K
通讯作者：
WADA, K
影响因子：
4.9
作者：
Kim, Joonyul;Smith, James J.;DellaPenna, Dean
通讯作者：
DellaPenna, Dean
影响因子：
5
作者：
Huang, Jun-Chao;Wang, Yan;Chen, Feng
通讯作者：
Chen, Feng
土豆块状类胡萝卜素的代谢工程通过七叶烯酸酯环化酶的块茎特异性沉默。
影响因子：
5.3
作者：
Diretto, Gianfranco;Tavazza, Raffaela;Welsch, Ralf;Pizzichini, Daniele;Mourgues, Fabienne;Papacchioli, Velia;Beyer, Peter;Giuliano, Giovanni
通讯作者：
Giuliano, Giovanni
DOI：
10.1073/pnas.241374598
发表时间：
2002-01-22
影响因子：
11.1
作者：
Fraser, PD;Romer, S;Bramley, PM
通讯作者：
Bramley, PM