Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase.
Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase.
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土豆块状类胡萝卜素的代谢工程通过七叶烯酸酯环化酶的块茎特异性沉默。
DOI：
10.1186/1471-2229-6-13
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发表时间：
2006-06-26
影响因子：
5.3
通讯作者：
Giuliano, Giovanni
中科院分区：
文献类型：
--
作者：
Diretto, Gianfranco;Tavazza, Raffaela;Welsch, Ralf;Pizzichini, Daniele;Mourgues, Fabienne;Papacchioli, Velia;Beyer, Peter;Giuliano, Giovanni
马铃薯是一种主要的主食，改变其维生素原的含量是减轻营养缺乏的一种可能的手段。β-胡萝卜素是维生素A的主要饮食前体。马铃薯块茎含有低水平的类胡萝卜素，主要由叶黄素、花药黄质、紫黄质和叶黄素酯组成。这些类胡萝卜素都没有维生素原A活性。我们沉默的第一个专门的步骤，在β-β-分支类胡萝卜素的生物合成，番茄红素环化酶（LCY-e），通过引入，通过农杆菌介导的转化，该基因的反义片段的马铃薯糖蛋白启动子的控制下。真实的时间测量证实了Lcy-e的结节特异性沉默。反义块茎显示β-β-类胡萝卜素水平显着增加，β-胡萝卜素显示最大的增加（高达14倍）。总类胡萝卜素增加了2.5倍。这些变化并不伴随着叶黄素的减少，这表明LCY-e对叶黄素积累没有速率限制。观察到通路中几个基因表达的特异性变化。这些数据表明，番茄红素的ε-环化是马铃薯块茎胡萝卜素生成的关键调控步骤。在相应基因的块茎特异性沉默后，β-β-类胡萝卜素和总类胡萝卜素水平增加，并且该途径中其他几个基因的表达被修饰。
Potato is a major staple food, and modification of its provitamin content is a possible means for alleviating nutritional deficiencies. beta-carotene is the main dietary precursor of vitamin A. Potato tubers contain low levels of carotenoids, composed mainly of the xanthophylls lutein, antheraxanthin, violaxanthin, and of xanthophyll esters. None of these carotenoids have provitamin A activity. We silenced the first dedicated step in the beta-epsilon- branch of carotenoid biosynthesis, lycopene epsilon cyclase (LCY-e), by introducing, via Agrobacterium-mediated transformation, an antisense fragment of this gene under the control of the patatin promoter. Real Time measurements confirmed the tuber-specific silencing of Lcy-e. Antisense tubers showed significant increases in beta-beta-carotenoid levels, with beta-carotene showing the maximum increase (up to 14-fold). Total carotenoids increased up to 2.5-fold. These changes were not accompanied by a decrease in lutein, suggesting that LCY-e is not rate-limiting for lutein accumulation. Tuber-specific changes in expression of several genes in the pathway were observed. The data suggest that epsilon-cyclization of lycopene is a key regulatory step in potato tuber carotenogenesis. Upon tuber-specific silencing of the corresponding gene, beta-beta-carotenoid and total carotenoid levels are increased, and expression of several other genes in the pathway is modified.
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影响因子：
46.9
作者：
Davuluri, GR;van Tuinen, A;Bowler, C
通讯作者：
Bowler, C
影响因子：
7.4
作者：
Hoa, TTC;Al-Babili, S;Beyer, P
通讯作者：
Beyer, P
影响因子：
5.1
作者：
Jorgensen, RA;Cluster, PD;Napoli, CA
通讯作者：
Napoli, CA
影响因子：
8.4
作者：
Morris, Wayne L.;Ducreux, Laurence J. M.;Taylor, Mark A.
通讯作者：
Taylor, Mark A.
影响因子：
7.2
作者：
Ralley, L;Enfissi, EMA;Fraser, PD
通讯作者：
Fraser, PD