Tracer-based estimates of protein flux in cases of incomplete product renewal: evidence and implications of heterogeneity in collagen turnover.
Tracer-based estimates of protein flux in cases of incomplete product renewal: evidence and implications of heterogeneity in collagen turnover.
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在产品更新不完全的情况下基于示踪剂的蛋白质通量估计：胶原蛋白周转异质性的证据和影响。
DOI：
10.1152/ajpendo.00435.2014
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kelley,DavidE
中科院分区：
文献类型：
--
作者：
Zhou,Haihong;Wang,Sheng-Ping;Herath,Kithsiri;Kasumov,Takhar;Sadygov,RovshanG;Previs,StephenF;Kelley,DavidE
各种分子的合成可以通过测量标记的前体掺入到感兴趣的产物中来估计。不幸的是，许多研究中的一个中心问题是无法估计前体的细胞内稀释度，从而正确计算产物的合成;通常认为测量真实的产物标记是简单的。我们启动了一项研究，以检查肝脏胶原蛋白的合成，并确定了一个明显的问题，与假设有关的测量产品标签。由于众所周知胶原蛋白的产生相对缓慢，因此我们依赖于[2 H]H2O标记（类似于预充输注）的使用，并在16天的过程中对动物进行采样。虽然水标记（前体）保持稳定，我们观察到标记的氨基酸掺入胶原蛋白，渐近蛋白质标记是相当低的预期的基础上的前体标记。虽然这种观察不一定令人惊讶（即，人们可能预期胶原蛋白库的相当大部分将呈现“惰性”或以非常慢的速率转变），其含义在某些领域是令人感兴趣的。在这里，我们讨论了一种新的情况下，示踪剂被用来量化的流量率的条件下，产品可能不会进行完全更换。我们演示了如何在产品池的异质性可以导致一个错误的结论，通量估计，我们概述了一种方法，可能有助于减少周围的数据解释的错误。
The synthesis of various molecules can be estimated by measuring the incorporation of a labeled precursor into a product of interest. Unfortunately, a central problem in many studies has been an inability to estimate the intracellular dilution of the precursor and therein correctly calculate the synthesis of the product; it is generally assumed that measuring the true product labeling is straightforward. We initiated a study to examine liver collagen synthesis and identified an apparent problem with assumptions regarding measurements of the product labeling. Since it is well known that collagen production is relatively slow, we relied on the use of [2H]H2O labeling (analogous to a primed infusion) and sampled animals over the course of 16 days. Although the water labeling (the precursor) remained stable and we observed the incorporation of labeled amino acids into collagen, the asymptotic protein labeling was considerably lower than what would be expected based on the precursor labeling. Although this observation is not necessarily surprising (i.e., one might expect that a substantial fraction of the collagen pool would appear “inert” or turn over at a very slow rate), its implications are of interest in certain areas. Herein, we discuss a novel situation in which tracers are used to quantify rates of flux under conditions where a product may not undergo complete replacement. We demonstrate how heterogeneity in the product pool can lead one to the wrong conclusions regarding estimates of flux, and we outline an approach that may help to minimize errors surrounding data interpretation.
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DOI：
10.1016/j.bbagen.2005.12.023
发表时间：
2006-05-01
影响因子：
3
作者：
Busch, Robert;Kim, Yoo-Kyeong;Hellerstein, Marc K.
通讯作者：
Hellerstein, Marc K.
以氚为示踪剂进行代谢周转的研究。
DOI：
10.1016/s0021-9258(18)55655-6
发表时间：
1956
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Ballou;R. Thompson
通讯作者：
R. Thompson
根据稳定同位素数据估计血浆载脂蛋白和脂质的分数合成率。
影响因子：
6.5
作者：
Foster,DM;Barrett,PH;Toffolo,G;Beltz,WF;Cobelli,C
通讯作者：
Cobelli,C
DOI：
10.1152/ajpendo.00579.2012
发表时间：
2013-04-01
影响因子：
5.1
作者：
Holm, Lars;O'Rourke, Bruce;Matthews, Dwight E.
通讯作者：
Matthews, Dwight E.
影响因子：
6.5
作者：
Alice H. Lichtenstein;J. S. Cohn;David L. Hachey;John S. Millar;J. Ordovás;Ernst J. Schaefer
通讯作者：
Alice H. Lichtenstein;J. S. Cohn;David L. Hachey;John S. Millar;J. Ordovás;Ernst J. Schaefer