Molecular analysis of the Aedes aegypti carboxypeptidase gene family.
Molecular analysis of the Aedes aegypti carboxypeptidase gene family.
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DOI：
10.1016/j.ibmb.2008.09.006
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发表时间：
2009-01
影响因子：
3.8
通讯作者：
Miesfeld, Roger L.
中科院分区：
文献类型：
--
作者：
Isoe, Jun;Zamora, Jorge;Miesfeld, Roger L.
为了更好地了解蚊虫中肠蛋白水解酶基因表达的协调调控，我们对埃及伊蚊消化羧肽酶进行了全面的分子研究。通过使用简并的聚合酶链式反应进行cDNA克隆和对最近完成的Ae.埃及伊蚊基因组，我们克隆并鉴定了18Ae。埃及伊蚊羧基肽酶基因。生物信息学分析表明，其中11个基因属于羧肽酶A家族(AaCPA-I至AaCPA-XI)，7个属于羧肽酶B基因家族(AaCPB-I至AaCPB-VII)。来自5个不同物种的32种蚊子羧基肽酶的系统发育分析表明，羧基肽酶基因家族的大部分序列分歧发生在伊蚊和按蚊的分离之前。不同于散布在Ae上的CPA基因。在埃及伊蚊基因组中，发现7个CPB基因中有6个位于一个120kb的基因组重叠群内，这表明它们很可能是由多个基因重复事件引起的。定量表达分析显示，11个Ae。埃及伊蚊的羧肽酶基因在中肠中被诱导高达40倍，根据基因的不同，峰值表达时间从摄食后3-36小时不等。
To gain a better understanding of coordinate regulation of protease gene expression in the mosquito midgut, we undertook a comprehensive molecular study of digestive carboxypeptidases in Aedes aegypti. Through a combination of cDNA cloning using degenerate PCR primers, and database mining of the recently completed Ae. aegypti genome, we cloned and characterized 18 Ae. aegypti carboxypeptidase genes. Bioinformatic analysis revealed that 11 of these genes belong to the carboxypeptidase A family (AaCPA-I through AaCPA-XI), and seven to the carboxypeptidase B gene family (AaCPB-I through AaCPB-VII). Phylogenetic analysis of 32 mosquito carboxypeptidases from five different species indicated that most of the sequence divergence in the carboxypeptidase gene family occurred prior to the separation of Aedes and Anopheles mosquito lineages. Unlike the CPA genes that are scattered throughout the Ae. aegypti genome, six of seven CPB genes were found to be located within a single 120 kb genome contig, suggesting that they most likely arose from multiple gene duplication events. Quantitative expression analysis revealed that 11 of the Ae. aegypti carboxypeptidase genes were induced up to 40-fold in the midgut in response to blood meal feeding, with peak expression times ranging from 3-36 hours post-feeding depending on the gene.
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影响因子：
2.6
作者：
Barillas-Mury, C.;Wells, M.A.
通讯作者：
Wells, M.A.
DOI：
10.1073/pnas.88.23.10821
发表时间：
1991-12-01
影响因子：
11.1
作者：
CHO, WL;DEITSCH, KW;RAIKHEL, AS
通讯作者：
RAIKHEL, AS
影响因子：
2.6
作者：
Lavazec, C;Bonnet, S;Bourgouin, C
通讯作者：
Bourgouin, C
影响因子：
2.6
作者：
Edwards, MJ;Moskalyk, LA;Jacobs-Lorena, M
通讯作者：
Jacobs-Lorena, M
DOI：
10.1073/pnas.97.20.10895
发表时间：
2000-09-26
影响因子：
11.1
作者：
Moreira, LA;Edwards, MJ;Jacobs-Lorena, M
通讯作者：
Jacobs-Lorena, M