Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocompsosite Membrane
Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocompsosite Membrane
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MG63细胞在羟基磷灰石/胶原纳米复合膜上的成骨活性
DOI：
10.4028/www.scientific.net/kem.330-332.313
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
M. Kikuchi
中科院分区：
文献类型：
--
作者：
M. Kikuchi
以羟基磷灰石/胶原（HAp/Col）纳米复合纤维为原料，制备了HAp/Col纳米复合膜。通过真空加热交联的HAp/Col膜显示出14±2（干）MPa和1.0±0.1（湿）MPa的拉伸强度，其强度足以在细胞培养和外科手术中操作。组织学观察和DNA总量分析表明，人成骨肉瘤细胞MG 63在HAp/Col膜上的粘附和增殖与在组织培养聚苯乙烯（TCPS）上的粘附和增殖相同。实时聚合酶链反应分析表明，HAp/Col膜上的碱性磷酸酶基因表达是TCPS上的1.4倍。这些结果表明，HAp/Col纳米复合膜可以用于骨组织工程支架以及骨填充物。
The hydroxyapatite/collagen (HAp/Col) nanocomposite membrane was prepared from the HAp/Col nanocomposite fibers. The HAp/Col membrane crosslinked by a vacuum heating showed 14±2 (dry) and 1.0±0.1 (wet) MPa in tensile strengths that were enough strength to operate in both cell culture and surgical operations. Histological observation and total DNA amount analysis demonstrated that human osteoblastic cells, MG63, derived from osteosarcoma on the HAp/Col membrane adhered and proliferated well as the same as those on tissue culture polystyrene (TCPS). Real-time polymerase chain reaction analyses indicated that alkaline phosphatase gene expression on the HAp/Col membrane was 1.4 times greater than that on TCPS. These results suggested that the HAp/Col nanocomposite membrane can be utilized in bone tissue engineering scaffold as well as bone filler.
DOI：
10.4028/www.scientific.net/kem.254-256.561
发表时间：
2003-12
期刊：
Key Engineering Materials
影响因子：
--
作者：
M. Kikuchi;T. Ikoma;D. Syoji;H. Matsumoto;Y. Koyama;S. Itoh;K. Takakuda;K. Shinomiya;M. Tanaka
通讯作者：
M. Kikuchi;T. Ikoma;D. Syoji;H. Matsumoto;Y. Koyama;S. Itoh;K. Takakuda;K. Shinomiya;M. Tanaka
羟基磷灰石/胶原自组织复合材料的纤维化
DOI：
--
发表时间：
2003
期刊：
Key Eng.Mat. 240-242
影响因子：
--
作者：
Kikuchi M.;Itoh S.;Matsumoto H.N.;Koyama;Y.;Takakuda K.;Shinomiya;K.;Tanaka J.
通讯作者：
Tanaka J.
影响因子：
14
作者：
Kikuchi, M;Itoh, S;Tanaka, J
通讯作者：
Tanaka, J