Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men.
Prolonged exposure to particulate pollution, genes associated with glutathione pathways, and DNA methylation in a cohort of older men.
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长时间暴露于颗粒污染，与谷胱甘肽途径相关的基因以及一群老年人中的DNA甲基化。
DOI：
10.1289/ehp.1002773
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发表时间：
2011-07
影响因子：
10.4
通讯作者：
Schwartz J
中科院分区：
文献类型：
--
作者：
Madrigano J;Baccarelli A;Mittleman MA;Wright RO;Sparrow D;Vokonas PS;Tarantini L;Schwartz J
背景：DNA甲基化是将环境暴露与疾病联系起来的潜在途径。暴露于颗粒空气污染与心血管发病率和死亡率增加有关，并且在与心血管发病率相关的过程中发现了较低的血液DNA甲基化。目的：我们假设长时间暴露于颗粒污染中可能与重复DNA元件的低甲基化有关，并且这种关联可能被参与谷胱甘肽代谢和其他宿主特征的基因修饰。方法：采用定量聚合酶链反应法对706名老年人的1406份血液样本进行DNA甲基化检测，检测长穿插核苷酸元件-1 （LINE-1）和短穿插核苷酸元件Alu的DNA甲基化。我们使用混合模型估计了与环境颗粒（空气动力学直径≤2.5µm的颗粒物质）、黑碳（BC）和硫酸盐（SO4）相关的重复元件DNA甲基化变化。在DNA甲基化测量之前，我们检查了多个暴露窗口（1-6个月）。我们研究了这种关联是否受到基因型和表型的修饰。结果：90天内BC的四分位数范围（IQR）增加与Alu中5-甲基胞嘧啶（5mC）减少0.31%（95%置信区间，0.12-0.50%）相关。在90天的时间内，SO4的IQR增加与LINE-1的0.27% 5mC（0.02-0.52%）下降相关。谷胱甘肽s -转移酶mu-1基因型增强了BC与Alu低甲基化之间的关联。结论：长期暴露于BC和SO4颗粒与两种重复元素的低甲基化有关。
Background: DNA methylation is a potential pathway linking environmental exposures to disease. Exposure to particulate air pollution has been associated with increased cardiovascular morbidity and mortality, and lower blood DNA methylation has been found in processes related to cardiovascular morbidity. Objective: We hypothesized that prolonged exposure to particulate pollution would be associated with hypomethylation of repetitive DNA elements and that this association would be modified by genes involved in glutathione metabolism and other host characteristics. Methods: DNA methylation of the long interspersed nucleotide element–1 (LINE-1) and the short interspersed nucleotide element Alu were measured by quantitative polymerase chain reaction pyrosequencing in 1,406 blood samples from 706 elderly participants in the Normative Aging Study. We estimated changes in repetitive element DNA methylation associated with ambient particles (particulate matter ≤ 2.5 µm in aerodynamic diameter), black carbon (BC), and sulfates (SO4), with mixed models. We examined multiple exposure windows (1–6 months) before DNA methylation measurement. We investigated whether this association was modified by genotype and phenotype. Results: An interquartile range (IQR) increase in BC over a 90-day period was associated with a decrease of 0.31% 5-methylcytosine (5mC) (95% confidence interval, 0.12–0.50%) in Alu. An IQR increase in SO4 over a 90-day period was associated with a decrease of 0.27% 5mC (0.02–0.52%) in LINE-1. The glutathione S-transferase mu-1–null genotype strengthened the association between BC and Alu hypomethylation. Conclusion: Prolonged exposure to BC and SO4 particles was associated with hypomethylation of two types of repetitive elements.
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影响因子：
3.6
作者：
Baccarelli A;Bollati V
通讯作者：
Bollati V
影响因子：
11.2
作者：
Bollati, Valentina;Baccarelli, Andrea;Yang, Allen S.
通讯作者：
Yang, Allen S.
DOI：
10.1097/ede.0b013e3181f20457
发表时间：
2010-11
期刊：
Epidemiology (Cambridge, Mass.)
影响因子：
--
作者：
Baccarelli A;Wright R;Bollati V;Litonjua A;Zanobetti A;Tarantini L;Sparrow D;Vokonas P;Schwartz J
通讯作者：
Schwartz J
影响因子：
30.8
作者：
Kazazian, HH;Moran, JV
通讯作者：
Moran, JV
影响因子：
4.7
作者：
Belinsky, SA;Snow, SS;Palmisano, WA
通讯作者：
Palmisano, WA