Embryonic stem cell-derived cardiomyocytes harbor a subpopulation of niche-forming Sca-1+ progenitor cells.
Embryonic stem cell-derived cardiomyocytes harbor a subpopulation of niche-forming Sca-1+ progenitor cells.
复制标题
DOI：
10.1007/s11010-010-0661-9
复制
发表时间：
2011-03
影响因子：
4.3
通讯作者：
Claycomb, William C.
中科院分区：
文献类型：
--
作者：
Lam, May L.;Hashem, Sherin I.;Claycomb, William C.
已知成年哺乳动物心脏含有心脏祖细胞群体。然而，这些细胞是作为心脏发育程序的一部分形成的，还是通过循环系统迁移到那里的，还没有明确的确定。进行这项研究是为了确定这一细胞群体的起源。利用遗传选择技术从小鼠胚胎干细胞（ES细胞）中建立心肌细胞群。为了确定在该ES细胞来源的心肌细胞群中是否存在心脏祖细胞，使用针对干细胞抗原-1（Sca-1）的抗体通过荧光激活细胞分选（FACS）分析细胞。我们观察到大约4%的心肌细胞群由Sca-1+细胞组成。当Sca-1+细胞通过磁性细胞分选分离并分化为细胞聚集体时，在100%的聚集体中观察到收缩。使用定量RT-PCR的基因表达研究表明，这些细胞表达终末分化的心脏特异性基因。当ES细胞衍生的心肌细胞与成人HL-1心肌细胞共培养形成三维细胞聚集体时，发现Sca-1+细胞“分选”并在细胞聚集体内形成小生境。我们的数据表明，心脏祖细胞在成人心脏起源的心脏发育计划的一部分，Sca-1+祖细胞可以提供一个重要的体外模型系统，以研究在心脏细胞龛的形成。
The adult mammalian heart is known to contain a population of cardiac progenitor cells. It has not been unambiguously determined, however, whether these cells form as part of the developmental program of the heart or migrate there by way of the circulatory system. This study was done in order to determine the origin of this population of cells. A population of cardiomyocytes was established from mouse embryonic stem (ES) cells using a genetic selection technique. In order to determine whether cardiac progenitor cells exist within this ES cell-derived cardiomyocyte population, the cells were analyzed by fluorescence activated cell sorting (FACS) using an antibody directed against stem cell antigen-1 (Sca-1). We observed that approximately 4% of the cardiomyocyte population was composed of Sca-1+ cells. When the Sca-1+ cells were isolated by magnetic cell sorting and differentiated as cellular aggregates, contractions were observed in 100% of the aggregates. Gene expression studies using quantitative RT-PCR showed that these cells expressed terminally differentiated cardiac-specific genes. When three-dimensional cellular aggregates were formed from ES cell-derived cardiomyocytes co-cultured with adult HL-1 cardiomyocytes, the Sca-1+ cells were found to “sort out” and form niches within the cell aggregates. Our data demonstrate that cardiac progenitor cells in the adult heart originate as part of the developmental program of the heart and that Sca-1+ progenitor cells can provide an important in vitro model system to study the formation of cellular niches in the heart.
登录
查看更多内容
影响因子：
23.9
作者：
Fuchs E
通讯作者：
Fuchs E
影响因子：
9.3
作者：
CLAYCOMB, WC
通讯作者：
CLAYCOMB, WC
DOI：
10.1073/pnas.0903089106
发表时间：
2009-10-06
影响因子：
11.1
作者：
Hosoda, Toru;D'Amario, Domenico;Leri, Annarosa
通讯作者：
Leri, Annarosa
影响因子：
64.8
作者：
Laugwitz, KL;Moretti, A;Chien, KR
通讯作者：
Chien, KR
影响因子：
2
作者：
Goel, Rohit Kumar;Suri, Vaishali;Srivastava, Arti
通讯作者：
Srivastava, Arti