Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
复制标题
DOI：
10.1016/j.cell.2015.01.008
复制
发表时间：
2015-02-12
期刊：
影响因子：
64.5
通讯作者：
Heisenberg CP
中科院分区：
文献类型：
--
作者：
Ruprecht V;Wieser S;Callan-Jones A;Smutny M;Morita H;Sako K;Barone V;Ritsch-Marte M;Sixt M;Voituriez R;Heisenberg CP
三维阿米巴细胞迁移是许多发育和疾病相关过程的中心，如癌症转移。在这里，我们在早期斑马鱼胚胎中确定了一种独特的原型变形虫细胞迁移模式，称为稳定泡迁移。稳定泡细胞呈现不变的极化球状，具有特殊的迁移速度和持久性。通过生化或机械刺激增加肌球蛋白II的活性，祖细胞可以可逆地转化为稳定泡细胞，而不考虑其原始命运和运动特性。通过理论和实验的结合，我们发现，在稳定泡细胞中，皮质收缩性波动触发了向变形虫运动的随机转换，并且皮质流动和收缩性梯度之间的正反馈维持了稳定泡细胞的极化。我们进一步表明，皮层后流驱动稳定的泡细胞在各种粘附和非粘附环境中迁移，揭示了一种高度通用的变形虫迁移表型。收缩性驱动皮层网络不稳定驱动细胞快速极化细胞极化由皮质正反馈回路维持皮层流动驱动细胞在受限的三维环境中快速持续运动细胞皮层收缩力的内在波动触发运动行为的转换，使胚胎祖细胞获得快速持续的迁移模式在受限的三维环境中
3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype. Embryonic progenitor cells transform into a prototypic amoeboid migration mode Contractility driven cortical network instabilities drive rapid cell polarization Cell polarization is maintained by a positive cortical feedback loop Cortical flows drive fast and persistent cell motility in confined 3D environments Cell-intrinsic fluctuation in cortical contraction forces triggers the switch in motility behavior that allows embryonic progenitor cells to acquire a fast and persistent migratory mode in confined 3D environments
登录
查看更多内容
影响因子：
56.9
作者：
Behrndt, Martin;Salbreux, Guillaume;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
影响因子：
9.2
作者：
Montero, JA;Kilian, B;Heisenberg, CP
通讯作者：
Heisenberg, CP
影响因子：
64.8
作者：
Mayer, Mirjam;Depken, Martin;Grill, Stephan W.
通讯作者：
Grill, Stephan W.
影响因子：
7.5
作者：
Laemmermann, Tim;Sixt, Michael
通讯作者：
Sixt, Michael
影响因子：
3.3
作者：
Callan-Jones, A. C.;Voituriez, R.
通讯作者：
Voituriez, R.