Epigenetics. Restricted epigenetic inheritance of H3K9 methylation.
Epigenetics. Restricted epigenetic inheritance of H3K9 methylation.
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DOI：
10.1126/science.1260638
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发表时间：
2015-04-03
期刊：
影响因子：
--
通讯作者：
Allshire RC
中科院分区：
文献类型：
--
作者：
Audergon PN;Catania S;Kagansky A;Tong P;Shukla M;Pidoux AL;Allshire RC
翻译后组蛋白修饰被认为允许独立于相关DNA序列的不同染色质状态的表观遗传传递。H3K9甲基化是异染色质形成所必需的，然而，缺乏其表观遗传性的证明。裂变酵母有一个单一的H3K9甲基转移酶，Clr 4，指导所有H3K9甲基化和异染色质。利用可释放的拴系Clr4揭示了一个活跃的过程，从野生型细胞中的拴系位点快速擦除H3K9甲基化。然而，假定的组蛋白去甲基化酶Epe1的失活允许H3K9甲基化和沉默的染色质维持在拴系位点通过许多有丝分裂，并通过减数分裂，后释放拴系的Clr4转代。因此，H3K9甲基化是一种可遗传的表观遗传标记，其传播通常通过其主动去除来抵消，这防止了异染色质的未经授权的遗传。
Post-translational histone modifications are believed to allow the epigenetic transmission of distinct chromatin states, independently of associated DNA sequences. H3K9 methylation is essential for heterochromatin formation, however, a demonstration of its epigenetic heritability is lacking. Fission yeast has a single H3K9 methyltransferase, Clr4, that directs all H3K9 methylation and heterochromatin. Utilizing releasable tethered Clr4 reveals that an active process rapidly erases H3K9 methylation from tethering sites in wild-type cells. However, inactivation of the putative histone demethylase Epe1 allows H3K9 methylation and silent chromatin maintenance at the tethering site through many mitotic divisions, and transgenerationally through meiosis, after release of tethered Clr4. Thus, H3K9 methylation is a heritable epigenetic mark whose transmission is usually countered by its active removal, which prevents the unauthorised inheritance of heterochromatin.
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DOI：
10.1038/nrg3355
发表时间：
2013-02
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.4
作者：
Trewick, Sarah C.;Minc, Elsa;Antonelli, Richard;Urano, Takeshi;Allshire, Robin C.
通讯作者：
Allshire, Robin C.
影响因子：
4
作者：
Reyes-Turcu, Francisca E.;Grewal, Shiv I. S.
通讯作者：
Grewal, Shiv I. S.
影响因子：
7.2
作者：
Cheng X
通讯作者：
Cheng X
影响因子：
56.9
作者：
Nakayam, J;Rice, JC;Grewal, SIS
通讯作者：
Grewal, SIS