Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice
Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice
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痤疮丙酸杆菌诱导的特异性体液免疫反应可预防小鼠胸膜肺炎放线杆菌感染
DOI：
10.1128/cvi.00667-13
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发表时间：
2014-01
影响因子：
--
通讯作者：
Han, Wenyu
中科院分区：
文献类型：
--
作者：
Li, Linxi;Sun, Changjiang;Feng, Xin;Han, Wenyu
摘要猪传染性胸膜肺炎是由胸膜肺炎放线杆菌引起的一种传染性疾病，对养猪业的经济、生态和动物福利具有重大影响。痤疮丙酸杆菌是一种兼性厌氧革兰氏阳性棒状杆菌，广泛存在于正常健康成年动物体内。我们以前已经证明，痤疮丙酸杆菌可以预防A。猪胸膜肺炎感染。为了阐明这种效应的机制并鉴定新的A.在胸膜肺炎疫苗中，通过体外间接免疫荧光和调理吞噬测定来分析抗痤疮丙酸杆菌抗体在预防感染中的作用。在B细胞耗竭小鼠模型中证实了痤疮丙酸杆菌诱导的特异性体液免疫应答的作用。A.胸膜肺炎表现出与抗痤疮丙酸杆菌抗体水平高度显著的正等级相关。痤疮丙酸杆菌诱导的特异性抗体具有联合收割机A.胸膜肺炎并增加A.胸膜肺炎的吞噬作用。此外，在小鼠B细胞耗竭模型中的分析证实，由痤疮丙酸杆菌诱导的体液免疫应答在对A.胸膜肺炎感染本研究进一步阐明了痤疮丙酸杆菌预防A.本研究为研制猪传染性胸膜肺炎的异种疫苗提供了有益的依据。
ABSTRACT Porcine contagious pleuropneumonia, caused by Actinobacillus pleuropneumoniae, has a major impact on economics, ecology, and animal welfare in the pig-rearing industry. Propionibacterium acnes, a facultative anaerobic Gram-positive corynebacterium, exists widely in normal healthy adult animals. We have shown previously that P. acnes can prevent A. pleuropneumoniae infections in mice and pigs. To elucidate the mechanism of this effect and to identify novel A. pleuropneumoniae vaccines, the role of anti-P. acnes antibodies in preventing infection was analyzed by indirect immunofluorescence and opsonophagocytosis assays in vitro. The role of the specific humoral immune response induced by P. acnes was confirmed in a B cell depletion mouse model. The survival rates of mice challenged with A. pleuropneumoniae exhibited a highly significant positive rank correlation with the levels of anti-P. acnes antibodies. The specific antibodies induced by P. acnes had the ability to combine with A. pleuropneumoniae and increase opsonization of A. pleuropneumoniae for phagocytosis. Furthermore, analysis in the murine B cell depletion model confirmed that the humoral immune response induced by P. acnes played an important role in resistance to A. pleuropneumoniae infection. In this study, we further elucidated the reasons that P. acnes can prevent A. pleuropneumoniae infection, which provides useful evidence for the development of heterologous vaccines for the control of porcine contagious pleuropneumonia.
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DOI：
10.1271/bbb.70159
发表时间：
2007-08
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
Y. Jung;S. Matsumoto;M. Yamashita;Kosuke Tomimatsu;K. Teruya;Y. Katakura;S. Shirahata
通讯作者：
Y. Jung;S. Matsumoto;M. Yamashita;Kosuke Tomimatsu;K. Teruya;Y. Katakura;S. Shirahata
影响因子：
5.5
作者：
L. Lei;Changjiang Sun;S. Lu;Xin Feng;Jiaqi Wang;W. Han
通讯作者：
L. Lei;Changjiang Sun;S. Lu;Xin Feng;Jiaqi Wang;W. Han
肿瘤内注射痤疮丙酸杆菌的注射可通过增强Th1免疫反应来抑制恶性黑色素瘤。
影响因子：
3.7
作者：
Tsuda K;Yamanaka K;Linan W;Miyahara Y;Akeda T;Nakanishi T;Kitagawa H;Kakeda M;Kurokawa I;Shiku H;Gabazza EC;Mizutani H
通讯作者：
Mizutani H
影响因子：
3.1
作者：
Annette M. Schlageter;T. Kozel
通讯作者：
Annette M. Schlageter;T. Kozel
DOI：
10.1084/jem.20011983
发表时间：
2002-05-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Yoneyama H;Narumi S;Zhang Y;Murai M;Baggiolini M;Lanzavecchia A;Ichida T;Asakura H;Matsushima K
通讯作者：
Matsushima K