PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.
PDF1.5 Enhances Adaptation to Low Nitrogen Levels and Cadmium Stress.
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PDF1.5 增强对低氮水平和镉胁迫的适应
DOI：
10.3390/ijms221910455
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发表时间：
2021-09-28
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Wu Z;Liu D;Yue N;Song H;Luo J;Zhang Z
环境适应能力在植物生长发育中起着重要作用，但其具体机制尚不清楚。在这里，我们确定了拟南芥植物防御素1基因AtPDF1.5在适应低氮（LN）水平和镉（Cd）胁迫的参与。组织化学分析表明，AtPDF1.5主要在节和果柄中表达，且在LN和Cd胁迫下显著诱导表达。亚细胞定位分析表明AtPDF1.5定位于细胞壁和细胞质。AtPDF1.5的过表达显著增强了对LN和Cd胁迫的适应，并增强了金属元素的分布。AtPDF1.5的功能中断减少适应LN和镉胁迫和受损的金属分布。在LN条件下，硝酸盐转运体AtNRT1.5的表达上调。当AtPDF1.5过表达时，硝酸盐转运蛋白AtNRT1.8的表达下调，导致NO3−向芽的转运增强。在Cd胁迫下，AtPDF1.5调控了金属转运蛋白AtHMP07、AtNRAMP4、AtNRAMP1和AtHIPP3的表达，从而促进了Cd在地上部的积累。因此，AtPDF1.5可能参与信号物质的加工或传递，在Cd污染修复和LN适应中发挥重要作用。
Environmental acclimation ability plays a key role in plant growth, although the mechanism remains unclear. Here, we determined the involvement of Arabidopsis thaliana PLANT DEFENSIN 1 gene AtPDF1.5 in the adaptation to low nitrogen (LN) levels and cadmium (Cd) stress. Histochemical analysis revealed that AtPDF1.5 was mainly expressed in the nodes and carpopodium and was significantly induced in plants exposed to LN conditions and Cd stress. Subcellular localization analysis revealed that AtPDF1.5 was cell wall- and cytoplasm-localized. AtPDF1.5 overexpression significantly enhanced adaptation to LN and Cd stress and enhanced the distribution of metallic elements. The functional disruption of AtPDF1.5 reduced adaptations to LN and Cd stress and impaired metal distribution. Under LN conditions, the nitrate transporter AtNRT1.5 expression was upregulated. Nitrate transporter AtNRT1.8 expression was downregulated when AtPDF1.5 was overexpressed, resulting in enhanced transport of NO3− to shoots. In response to Cd treatment, AtPDF1.5 regulated the expression of metal transporter genes AtHMP07, AtNRAMP4, AtNRAMP1, and AtHIPP3, resulting in higher Cd accumulation in the shoots. We conclude that AtPDF1.5 is involved in the processing or transmission of signal substances and plays an important role in the remediation of Cd pollution and LN adaptation.
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DOI：
10.1073/pnas.1702975114
发表时间：
2017-04-18
影响因子：
11.1
作者：
Agorio, Astrid;Giraudat, Jerome;Merlot, Sylvain
通讯作者：
Merlot, Sylvain
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
16.6
作者：
Luo JS;Huang J;Zeng DL;Peng JS;Zhang GB;Ma HL;Guan Y;Yi HY;Fu YL;Han B;Lin HX;Qian Q;Gong JM
通讯作者：
Gong JM
影响因子：
7.2
作者：
Larsen, PB;Cancel, JD
通讯作者：
Cancel, JD
拟南芥 NRT1.5 是调节硝酸盐重新分配和胁迫耐受性的另一个重要组成部分
影响因子：
7.4
作者：
Chen, Chun-Zhu;Lv, Xin-Fang;Gong, Ji-Ming
通讯作者：
Gong, Ji-Ming