Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions.
Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions.
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DOI：
10.1038/srep35284
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发表时间：
2016-10-18
影响因子：
4.6
通讯作者：
Ben Mamoun C
中科院分区：
文献类型：
--
作者：
Silva JC;Cornillot E;McCracken C;Usmani-Brown S;Dwivedi A;Ifeonu OO;Crabtree J;Gotia HT;Virji AZ;Reynes C;Colinge J;Kumar V;Lawres L;Pazzi JE;Pablo JV;Hung C;Brancato J;Kumari P;Orvis J;Tretina K;Chibucos M;Ott S;Sadzewicz L;Sengamalay N;Shetty AC;Su Q;Tallon L;Fraser CM;Frutos R;Molina DM;Krause PJ;Ben Mamoun C
微巴贝斯虫是一种主要在小型哺乳动物中传播的红血球原生动物寄生虫，是人类巴贝斯虫病的主要病因。虽然大多数病例是由蜱虫传播的，但这种疾病也可能通过输血和围产期传播。对7株微孢子虫的基因组组成、遗传多样性和基因表达谱的综合分析表明，美国东北部分离物的遗传变异几乎完全与寄生虫表面蛋白质组和分泌组的编码基因相关。此外，我们发现多态性仅限于少数基因，这些基因在感染期间高度表达。为了鉴定参与宿主-寄生虫相互作用的病原体编码因子，我们筛选了一个由174个微螺旋体蛋白组成的蛋白质组阵列，其中包括一些寄生虫分泌组的预测成员。使用这种免疫蛋白质组学方法，我们确定了几种新的抗原，这些抗原在感染开始时触发强烈的宿主免疫反应。本文提供的基因组学和免疫学数据首次揭示了微孢子虫与其哺乳动物宿主相互作用的决定因素，以及它们与理解作用于寄生虫进化的选择压力的相关性。
Babesia microti, a tick-transmitted, intraerythrocytic protozoan parasite circulating mainly among small mammals, is the primary cause of human babesiosis. While most cases are transmitted by Ixodes ticks, the disease may also be transmitted through blood transfusion and perinatally. A comprehensive analysis of genome composition, genetic diversity, and gene expression profiling of seven B. microti isolates revealed that genetic variation in isolates from the Northeast United States is almost exclusively associated with genes encoding the surface proteome and secretome of the parasite. Furthermore, we found that polymorphism is restricted to a small number of genes, which are highly expressed during infection. In order to identify pathogen-encoded factors involved in host-parasite interactions, we screened a proteome array comprised of 174 B. microti proteins, including several predicted members of the parasite secretome. Using this immuno-proteomic approach we identified several novel antigens that trigger strong host immune responses during the onset of infection. The genomic and immunological data presented herein provide the first insights into the determinants of B. microti interaction with its mammalian hosts and their relevance for understanding the selective pressures acting on parasite evolution.
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影响因子：
3.2
作者：
Goethert, Heidi K.;Telford, Sam R., III
通讯作者：
Telford, Sam R., III
影响因子：
158.5
作者：
Krause, PJ;Spielman, A;Persing, DH
通讯作者：
Persing, DH
影响因子：
64.8
作者：
Elsworth, Brendan;Matthews, Kathryn;de Koning-Ward, Tania F.
通讯作者：
de Koning-Ward, Tania F.
影响因子：
3.3
作者：
Ifeonu, Olukemi O.;Chibucos, Marcus C.;Silva, Joana C.
通讯作者：
Silva, Joana C.
DOI：
10.1111/tra.12049
发表时间：
2013-05
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Hsiao CH;Luisa Hiller N;Haldar K;Knoll LJ
通讯作者：
Knoll LJ