Mesenchymal stem cells derived from adipose tissue vs bone marrow: in vitro comparison of their tropism towards gliomas.
Mesenchymal stem cells derived from adipose tissue vs bone marrow: in vitro comparison of their tropism towards gliomas.
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DOI：
10.1371/journal.pone.0058198
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Quinones-Hinojosa A
中科院分区：
文献类型：
--
作者：
Pendleton C;Li Q;Chesler DA;Yuan K;Guerrero-Cazares H;Quinones-Hinojosa A
胶质母细胞瘤是最常见的原发性恶性脑肿瘤，难以手术切除、放疗和化疗。人间充质干细胞（hMSC）可以从骨髓（BMSC）和脂肪（AMSC）组织中获得。这些细胞是研究辅助治疗递送的一个有希望的途径。尽管对骨髓间充质干细胞趋向性的推测机制进行了广泛的研究，但目前还没有直接比较骨髓间充质干细胞和骨髓间充质干细胞对胶质瘤的趋向性的疗效和特异性。在irb批准的方案下，术中建立了人脂肪间充质干细胞（hAMSCs），并对其细胞表面标记物进行了表征，这些标记物来源于间充质干细胞，并具有三谱系分化（成脂、成软骨和成骨）的潜力。经过验证的实验hAMSCs与商业衍生的hBMSCs （Lonza）和hAMSCs （Invitrogen）在对原发胶质瘤神经球培养获得的胶质瘤条件介质的生长反应性和胶质瘤亲和性方面进行了比较。商业和原代培养的AMSCs和商业BMSCs在体外向胶质瘤条件培养基迁移方面没有统计学上的显著差异。与原代培养的AMSCs相比，商业AMSCs和BMSCs的增殖率有统计学差异，这表明原代培养比市售的细胞系生长速度慢。脂肪和骨髓来源的间充质干细胞在体外具有相似的胶质瘤倾向。鉴于在局部麻醉下收获大量间充质干细胞的能力，脂肪组织可能为研究和临床应用提供更有效的间充质干细胞来源，同时最大限度地减少细胞收获过程中的患者发病率。
Glioblastoma is the most common primary malignant brain tumor, and is refractory to surgical resection, radiation, and chemotherapy. Human mesenchymal stem cells (hMSC) may be harvested from bone marrow (BMSC) and adipose (AMSC) tissue. These cells are a promising avenue of investigation for the delivery of adjuvant therapies. Despite extensive research into putative mechanisms for the tumor tropism of MSCs, there remains no direct comparison of the efficacy and specificity of AMSC and BMSC tropism towards glioma. Under an IRB-approved protocol, intraoperative human Adipose MSCs (hAMSCs) were established and characterized for cell surface markers of mesenchymal stem cell origin in conjunction with the potential for tri-lineage differentiation (adipogenic, chondrogenic, and osteogenic). Validated experimental hAMSCs were compared to commercially derived hBMSCs (Lonza) and hAMSCs (Invitrogen) for growth responsiveness and glioma tropism in response to glioma conditioned media obtained from primary glioma neurosphere cultures. Commercial and primary culture AMSCs and commercial BMSCs demonstrated no statistically significant difference in their migration towards glioma conditioned media in vitro. There was statistically significant difference in the proliferation rate of both commercial AMSCs and BMSCs as compared to primary culture AMSCs, suggesting primary cultures have a slower growth rate than commercially available cell lines. Adipose- and bone marrow-derived mesenchymal stem cells have similar in vitro glioma tropism. Given the well-documented ability to harvest larger numbers of AMSCs under local anesthesia, adipose tissue may provide a more efficient source of MSCs for research and clinical applications, while minimizing patient morbidity during cell harvesting.
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