Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand.
Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand.
复制标题
DOI：
10.1186/1475-2875-5-121
复制
发表时间：
2006-12-14
期刊：
影响因子：
3
通讯作者：
Khuntirat B
中科院分区：
文献类型：
--
作者：
Coleman RE;Sattabongkot J;Promstaporm S;Maneechai N;Tippayachai B;Kengluecha A;Rachapaew N;Zollner G;Miller RS;Vaughan JA;Thimasarn K;Khuntirat B
The main objective of this study was to compare the performance of nested PCR with expert microscopy as a means of detecting Plasmodium parasites during active malaria surveillance in western Thailand. The study was performed from May 2000 to April 2002 in the village of Kong Mong Tha, located in western Thailand. Plasmodium vivax (PV) and Plasmodium falciparum (PF) are the predominant parasite species in this village, followed by Plasmodium malariae (PM) and Plasmodium ovale (PO). Each month, fingerprick blood samples were taken from each participating individual and used to prepare thick and thin blood films and for PCR analysis. PCR was sensitive (96%) and specific (98%) for malaria at parasite densities ≥ 500/μl; however, only 18% (47/269) of P. falciparum- and 5% (20/390) of P. vivax-positive films had parasite densities this high. Performance of PCR decreased markedly at parasite densities <500/μl, with sensitivity of only 20% for P. falciparum and 24% for P. vivax at densities <100 parasites/μl. Although PCR performance appeared poor when compared to microscopy, data indicated that the discrepancy between the two methods resulted from poor performance of microscopy at low parasite densities rather than poor performance of PCR. These data are not unusual when the diagnostic method being evaluated is more sensitive than the reference method. PCR appears to be a useful method for detecting Plasmodium parasites during active malaria surveillance in Thailand.
登录
查看更多内容
DOI：
10.4269/ajtmh.1996.55.647
发表时间：
1996-12-01
影响因子：
3.3
作者：
Jelinek, T;Proll, S;Kilian, AHD
通讯作者：
Kilian, AHD
影响因子：
9.4
作者：
Zhong, KJY;Salas, CJ;Kain, KC
通讯作者：
Kain, KC
影响因子：
9.4
作者：
Perandin, F;Manca, N;Chezzi, C
通讯作者：
Chezzi, C
影响因子：
1.5
作者：
SNOUNOU, G;VIRIYAKOSOL, S;BROWN, KN
通讯作者：
BROWN, KN
DOI：
10.4269/ajtmh.1999.60.183
发表时间：
1999-02-01
影响因子：
3.3
作者：
Rubio, JM;Benito, A;Alvar, J
通讯作者：
Alvar, J