Genome-wide analysis of the NAAT, DMAS, TOM, and ENA gene families in maize suggests their roles in mediating iron homeostasis.
Genome-wide analysis of the NAAT, DMAS, TOM, and ENA gene families in maize suggests their roles in mediating iron homeostasis.
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对玉米中 NAAT、DMAS、TOM 和 ENA 基因家族的全基因组分析表明它们在介导铁稳态中的作用。
DOI：
10.1186/s12870-021-03422-7
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发表时间：
2022-01-17
影响因子：
5.3
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Zhang X;Xiao K;Li S;Li J;Huang J;Chen R;Pang S;Zhou X
烟酰胺（NA）、2′-脱氧麦根酸（DMA）和麦根酸（MA）是植物吸收和运输铁所必需的螯合剂。烟草胺氨基转移酶（NAAT）、2′-脱氧麦黄酸合成酶（DMAS）、MAs转运蛋白（TOM）和NA外排转运蛋白（ENA）参与水稻、小麦和大麦的铁吸收和转运，但这些家族在玉米中尚未完全鉴定和全面分析。在这里，我们通过基因组挖掘确定了5个ZmNAAT，9个ZmDMAS，11个ZmTOM和2个ZmENA基因。RNA测序和定量实时PCR分析表明，这些基因在各种组织中表达，并对高铁和低铁条件作出不同的反应。特别是，缺铁刺激ZmDMAS 1，ZmTOM 1，ZmTOM 3和ZmENA 1的表达。此外，我们确定蛋白质的亚细胞定位的瞬时表达的绿色荧光蛋白融合在玉米叶肉原生质体。ZmNAAT 1、ZmNAAT-L4、ZmDMAS 1和ZmDMAS-L1定位于细胞质中，而ZmTOMs和ZmENA靶向质膜和液泡膜、内膜和囊泡。我们的研究结果表明，ZmNAAT，ZmDMAS，ZmTOM和ZmENA家族成员的不同基因表达谱和亚细胞定位可能使植物铁载体代谢在不同组织和不同的外部条件下的特异性调节，揭示在玉米中的铁稳态，并提供候选基因的育种富铁玉米品种。在线版本包含补充材料，可通过10.1186/s12870-021-03422-7获得。
Nicotianamine (NA), 2′-deoxymugineic acid (DMA), and mugineic acid (MA) are chelators required for iron uptake and transport in plants. Nicotianamine aminotransferase (NAAT), 2′-deoxymugineic acid synthase (DMAS), transporter of MAs (TOM), and efflux transporter of NA (ENA) are involved in iron uptake and transport in rice (Oryza sativa), wheat (Triticum aestivum), and barley (Hordeum vulgare); however, these families have not been fully identified and comprehensively analyzed in maize (Zea mays L.). Here, we identified 5 ZmNAAT, 9 ZmDMAS, 11 ZmTOM, and 2 ZmENA genes by genome mining. RNA-sequencing and quantitative real-time PCR analysis revealed that these genes are expressed in various tissues and respond differently to high and low iron conditions. In particular, iron deficiency stimulated the expression of ZmDMAS1, ZmTOM1, ZmTOM3, and ZmENA1. Furthermore, we determined protein subcellular localization by transient expression of green fluorescent protein fusions in maize mesophyll protoplasts. ZmNAAT1, ZmNAAT-L4, ZmDMAS1, and ZmDMAS-L1 localized in the cytoplasm, whereas ZmTOMs and ZmENAs targeted to plasma and tonoplast membranes, endomembranes, and vesicles. Our results suggest that the different gene expression profiles and subcellular localizations of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA family members may enable specific regulation of phytosiderophore metabolism in different tissues and under different external conditions, shedding light on iron homeostasis in maize and providing candidate genes for breeding iron-rich maize varieties. The online version contains supplementary material available at 10.1186/s12870-021-03422-7.
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影响因子：
4.9
作者：
Kakei Y;Yamaguchi I;Kobayashi T;Takahashi M;Nakanishi H;Yamakawa T;Nishizawa NK
通讯作者：
Nishizawa NK
影响因子：
5.6
作者：
Díaz-Benito P;Banakar R;Rodríguez-Menéndez S;Capell T;Pereiro R;Christou P;Abadía J;Fernández B;Álvarez-Fernández A
通讯作者：
Álvarez-Fernández A
影响因子：
7.4
作者：
Haydon, Michael J.;Cobbett, Christopher S.
通讯作者：
Cobbett, Christopher S.
影响因子：
7.4
作者：
Klatte, Marco;Schuler, Mara;Bauer, Petra
通讯作者：
Bauer, Petra
影响因子：
5.6
作者：
Jain A;Connolly EL
通讯作者：
Connolly EL