Generating nonlinear concentration gradients in microfluidic devices for cell studies.
Generating nonlinear concentration gradients in microfluidic devices for cell studies.
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DOI：
10.1021/ac2001737
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发表时间：
2011-03-15
影响因子：
7.4
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Selimovic, Seila;Sim, Woo Young;Kirn, Sang Bok;Jang, Yun Ho;Lee, Won Gu;Khabiry, Masoud;Bae, Hojae;Jambovane, Sachin;Hong, Jong Wook;Khademhosseini, Ali
我们描述了一种用于产生非线性（指数和S形）浓度梯度的微流体装置，该装置与用于细胞储存和分析的微孔阵列相结合。该装置具有用于使多种水溶液共流的两个输入端，主共流通道和流体通道的不对称网格，其允许两种溶液在交叉点处联合收割机结合而不完全混合。由于这两种物质在共流通道中的不对称性和扩散，不同数量的两种溶液进入每个流体路径，这分别在低流速和高流速下诱导指数和S形浓度梯度，使得微流体装置通用。这种设计的一个关键特点是节省空间，因为它不需要多路复用或单独的混合通道阵列。此外，梯度结构可以与细胞实验一起使用，以将微孔中捕获的细胞暴露于各种浓度的可溶性因子。我们证明了这种设计的实用性，以评估成纤维细胞的活力，在一系列的过氧化氢（H2O2）浓度。
We describe a microfluidic device for generating non-linear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for co-flowing multiple aqueous solutions, a main co-flow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the co-flow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space-saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H2O2) concentrations.
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DOI：
10.1073/pnas.0509483102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gregor, T;Bialek, W;Wieschaus, EF
通讯作者：
Wieschaus, EF
影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
29.4
作者：
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通讯作者：
Langer, R
影响因子：
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作者：
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通讯作者：
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