Detection of DOPA-melanin in the dimorphic fungal pathogen Penicillium marneffei and its effect on macrophage phagocytosis in vitro.
Detection of DOPA-melanin in the dimorphic fungal pathogen Penicillium marneffei and its effect on macrophage phagocytosis in vitro.
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二相性真菌病原菌马尔尼菲青霉中多巴黑色素的检测及其对体外巨噬细胞吞噬作用的影响。
DOI：
10.1371/journal.pone.0092610
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tan W
中科院分区：
文献类型：
--
作者：
Liu D;Wei L;Guo T;Tan W
真菌病原体马尔尼菲青霉在体外产生黑色素样色素。黑色素的合成途径及其对巨噬细胞内存活的保护性酵母细胞的可能影响尚不清楚。在这项工作中，马尔尼菲酵母在 L-DOPA 存在下产生棕黑色素，并从用蛋白水解酶、变性剂和浓热酸处理的酵母细胞中提取黑色颗粒。曲酸抑制在脑心浸液琼脂上生长的马尔尼菲酵母的棕黑色素产生。透射电子显微镜显示，最内层细胞壁中呈珠状排列，平均直径为 100 nm 的球形粒状电子致密颗粒。电子顺磁共振显示黑色颗粒含有稳定的自由基化合物。从马尔尼菲 P. marneffei 中提取的颗粒和合成的多巴黑色素的紫外可见光谱和傅里叶变换红外光谱显示出高度的相似性。黑色化的酵母细胞降低了巨噬细胞的吞噬作用，并增加了对体外细胞内消化的抵抗力。这些结果表明马尔尼菲原菌可以在体外合成多巴黑色素或黑色素样化合物，并表明多巴黑色素途径与细胞壁结构相关并增强巨噬细胞对吞噬作用的抵抗力。
The fungal pathogen Penicillium marneffei produces melanin-like pigment in vitro. The synthetic pathway of melanin and its possible influence in the protective yeast cells surviving within macrophage cells are not known. In this work, P. marneffei produced brown black pigment in the presence of L-DOPA and black particles were extracted from yeast cells treated with proteolytic enzymes, denaturant and concentrated hot acid. Kojic acid inhibited the brown-black pigment production of P. marneffei yeast grown on brain heart infusion agar. Transmitting electron microscopy showed spherical granular electron-dense particles with an average diameter of 100 nm in a beaded arrangement in the innermost cell wall. Electron-paramagnetic resonance revealed that the black particles contain a stable free radical compound. The UV-visible and Fourier transform infrared spectra of particles extracted from P. marneffei and synthetic DOPA-melanin showed a high degree of similarity. Melanized yeast cells decreased phagocytosis by macrophage cells and increased resistance to intracellular digestion in vitro. These results indicate that P. marneffei can synthesize DOPA-melanin or melanin-like compounds in vitro and suggest that the DOPA-melanin pathway is associated with cell wall structure and enhances the resistance to phagocytosis by macrophages.
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影响因子：
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