Aspergillus fumigatus conidial melanin modulates host cytokine response.
Aspergillus fumigatus conidial melanin modulates host cytokine response.
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DOI：
10.1016/j.imbio.2009.10.002
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发表时间：
2010-11
期刊：
影响因子：
2.8
通讯作者：
Kullberg, Bart Jan
中科院分区：
文献类型：
--
作者：
Chai, Louis Y. A.;Netea, Mihai G.;Sugui, Janyce;Vonk, Alieke G.;van de Sande, Wendy W. J.;Warris, Adilia;Kwon-Chung, Kyung J.;Kullberg, Bart Jan
黑色素生物色素与真菌毒力有关。烟曲霉分生孢子是黑化的并且是弱免疫原性的。我们发现，黑色素表面上的休息烟曲霉分生孢子可能有助于掩盖病原体相关的分子模式（PAMPs）诱导的细胞因子反应。与黑化的野生型分生孢子相比，白化病分生孢子在人外周血单核细胞（PBMC）中诱导显著更多的促炎细胞因子。阻断dectin-1受体、Toll样受体4或甘露糖受体可降低由白化病诱导的细胞因子产生，但对野生型分生孢子无影响。此外，与从健康供体分离的细胞的刺激相比，白化病分生孢子刺激从具有缺陷的dectin-1的个体分离的PBMC中的细胞因子产生的效力较低。这些结果表明，β-葡聚糖以及其他刺激性PAMP如甘露聚糖衍生物在不存在黑色素的情况下暴露在分生孢子表面上。黑色素可能通过阻碍宿主免疫细胞对A.烟熏。
Melanin biopigments have been linked to fungal virulence. Aspergillus fumigatus conidia are melanised and are weakly immunogenic. We show that melanin pigments on the surface of resting Aspergillus fumigatus conidia may serve to mask pathogen-associated molecular patterns (PAMPs)-induced cytokine response. The albino conidia induced significantly more proinflammatory cytokines in human peripheral blood mononuclear cells (PBMC), as compared to melanised wild-type conidia. Blocking dectin-1 receptor, Toll-like receptor 4 or mannose receptor decreased cytokine production induced by the albino but not by the wild type conidia. Moreover, albino conidia stimulated less potently, cytokine production in PBMC isolated from an individual with defective dectin-1, compared to the stimulation of cells isolated from healthy donors. These results suggest that β-glucans, but also other stimulatory PAMPs like mannan derivatives, are exposed on conidial surface in the absence of melanin. Melanin may play a modulatory role by impeding the capability of host immune cells to respond to specific ligands on A. fumigatus.
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DOI：
10.1086/523110
发表时间：
2007-11-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Gow NA;Netea MG;Munro CA;Ferwerda G;Bates S;Mora-Montes HM;Walker L;Jansen T;Jacobs L;Tsoni V;Brown GD;Odds FC;Van der Meer JW;Brown AJ;Kullberg BJ
通讯作者：
Kullberg BJ
影响因子：
3.2
作者：
LEHRER, RI;CLINE, MJ
通讯作者：
CLINE, MJ
影响因子：
3.2
作者：
Tsai, HF;Chang, YC;Kwon-Chung, KJ
通讯作者：
Kwon-Chung, KJ
影响因子：
5.4
作者：
Cambi, A;Gijzen, K;Figdor, CG
通讯作者：
Figdor, CG
影响因子：
3.4
作者：
Luther, Kathrin;Torosantucci, Antonella;Ebel, Frank
通讯作者：
Ebel, Frank