Laminarin enhances the activity of natural killer cells in immunosuppressed mice
Laminarin enhances the activity of natural killer cells in immunosuppressed mice
复制标题
海带多糖增强免疫抑制小鼠自然杀伤细胞的活性
DOI：
10.5114/ceji.2019.92784
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hong Yu
中科院分区：
文献类型：
--
作者：
Xiaobao Zhu;R. Zhu;Zhiyuan Jian;Hong Yu
本研究旨在探讨层粘连蛋白对免疫抑制小鼠自然杀伤细胞（NK）毒性的影响及其机制。采用环磷酰胺（cy）制备小鼠免疫抑制模型。两组小鼠每天分别灌胃层叠肽500 mg/kg和1000 mg/kg，连续10 d。采用MACS法分离脾脏NK细胞，在体内检测NK细胞的细胞毒性及血清中IL-12、IFN-γ水平。体外检测NK92-MI细胞的细胞毒性、激活受体（NKp30、NKp44、NKp46和NKG2D）以及穿孔素和颗粒酶B的表达。与正常对照组相比，cy模型组大鼠血清NK细胞毒性、IL-12、IFN-γ水平均显著降低（p < 0.01）。与cy模型组相比，层压肽显著提高了NK细胞的细胞毒性，血清中IL-12和IFN-γ水平（p < 0.05）。在体外，层粘连素增加了NK92-MI细胞的细胞毒性、NKp30和NKG2D、穿孔素和颗粒酶B的表达（p < 0.01）。本研究表明，层粘菌素可通过提高血清IL-12和IFN-γ水平以及NKp30和NKG2D、穿孔素和颗粒酶B的表达，促进免疫抑制小鼠NK细胞的细胞毒性。
The aim of the study was to investigate the effects of laminarin on natural killer (NK) cell cytotoxicity of immunosuppressive mice and its mechanism. Cyclophosphamide (cy) was used to make an immunosuppressive model of mice. The mice of two groups were given interventions by gavage with laminarin 500 mg/kg and 1000 mg/kg every day for 10 days. MACS was adopted to isolate spleen NK cells, and cytotoxicity of NK cells and IL-12, IFN-γ level in serum were detected in vivo. Cytotoxicity of NK92-MI cells, activating receptors (NKp30, NKp44, NKp46 and NKG2D) and perforin and granzyme B expression were detected in vitro. Compared to the normal control group, the cytotoxicity of NK cells, IL-12 and IFN-γ level in serum in the cy model group were all reduced significantly (p < 0.01). Compared to the cy model group, laminarin increased the cytotoxicity of NK cells, IL-12 and IFN-γ levels in serum significantly (p < 0.05). In vitro, laminarin increased the cytotoxicity, NKp30 and NKG2D, perforin and granzyme B expressions of NK92-MI cells (p < 0.01). This research showed that laminarin can promote NK cell cytotoxicity in immunosuppressive mice by increasing the levels of IL-12 and IFN-γ in serum and expressions of NKp30 and NKG2D, perforin and granzyme B.
影响因子：
14.2
作者：
Campbell, Kerry S.;Hasegawa, Jun
通讯作者：
Hasegawa, Jun
影响因子：
--
作者：
C. Watzl
通讯作者：
C. Watzl
影响因子：
5.4
作者：
Kouakou, Koffi;Schepetkin, Igor A.;Yapi, Ahoua;Kirpotina, Liliya N.;Jutila, Mark A.;Quinn, Mark T.
通讯作者：
Quinn, Mark T.